Granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease.
Granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease.
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DOI：
10.1111/j.1600-0765.2008.01134.x
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发表时间：
2009-08
影响因子：
3.5
通讯作者：
Papapanou PN
中科院分区：
文献类型：
--
作者：
Kebschull M;Demmer R;Behle JH;Pollreisz A;Heidemann J;Belusko PB;Celenti R;Pavlidis P;Papapanou PN
粘膜炎症反应在很大程度上是由促炎趋化因子调控的。我们的目的是评估趋化因子GCP-2在临床健康和疾病牙龈组织中的表达，并探索与牙周炎的临床和微生物标志物的可能相关性。趋化因子粒细胞趋化蛋白2(GCP-2/CXCL6)参与了中性粒细胞的募集和迁移。以往的研究表明，GCP-2在黏膜炎症中表达上调，例如在炎症性肠病中，与功能和结构相关的趋化因子白介素8(IL-8)相似。然而，与IL-8不同的是，GCP-2在牙周炎中的作用尚未被证明。用Affymetrix U133Plus2.0芯片分析了90例牙周炎患者的184个“病态”和63个“健康”牙龈组织标本的基因表达。实时荧光定量RT-PCR、Western blotting和ELISA法进一步证实GCP-2在牙周组织中的表达，并用免疫组织化学方法分析GCP-2蛋白在牙周组织中的定位。从邻近牙周袋采集菌斑样本，用棋盘DNA-DNA杂交法对11种牙周菌斑进行鉴定。在所有已知的趋化因子中，GCP-2mRNA3.8倍(p<；1.1×10−16)在“疾病”与“健康”组织中表达最高，而IL-8mRNA2.6倍(p<；1.2×10−15)。GCP-2的表达增加与红色和橙色复合病原体的水平增加以及探测深度的增加相关，但与附着丧失无关。免疫组织化学显示GCP-2在牙周血管内皮细胞有表达。GCP-2与牙周炎的严重程度相关，在患病的牙龈组织中，GCP-2似乎是一种迄今未被认识的IL-8的功能辅助物。
Mucosal inflammatory responses are orchestrated largely by proinflammatory chemokines. We aimed to evaluate the expression of the chemokine GCP-2 in clinically healthy vs. diseased gingival tissues and to explore possible correlations with clinical and microbiological markers of periodontitis. The chemokine granulocyte chemotactic protein 2 (GCP-2/CXCL6) is involved in neutrophil recruitment and migration. Previous studies showed that GCP-2 is upregulated in mucosal inflammation, e.g., in inflammatory bowel disease, similarly to the functionally and structurally related chemokine interleukin 8 (IL-8). Nevertheless, unlike IL-8, a role of GCP-2 in gingival inflammation has not yet been demonstrated. Gene expression in 184 ‘diseased’ and 63 ‘healthy’ gingival tissue specimens from 90 periodontitis patients was analyzed using Affymetrix U133Plus2.0 arrays. GCP-2 expression was further confirmed by real time RT-PCR, western blotting and ELISA while the localization of GCP-2 protein in the gingival tissues was analyzed by immunohistochemistry. Plaque samples from the adjacent periodontal pockets were collected and evaluated for 11 periodontal species using checkerboard DNA-DNA hybridizations. Among all known chemokines, GCP-2 mRNA was the most expressed (3.8 fold, p<1.1×10−16) in ‘diseased’ vs. ‘healthy’ tissue, as compared to a 2.6 fold increased expression of IL-8 mRNA (p<1.2×10−15). Increased expression of GCP-2 correlated with higher levels of ‘red’ and ‘orange’ complex pathogens and with increased probing depth, but not with attachment loss. Immuno-histochemistry showed that GCP-2 was expressed in gingival vascular endothelium. GCP-2 correlates with the severity of periodontitis and appears to act as a hitherto unrecognized functional adjunct to IL-8 in diseased gingival tissues.
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