Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands.
Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands.
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DOI：
10.3201/eid2705.204055
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发表时间：
2021-05
影响因子：
11.8
通讯作者：
Egberink H
中科院分区：
文献类型：
--
作者：
Zhao S;Schuurman N;Li W;Wang C;Smit LAM;Broens EM;Wagenaar JA;van Kuppeveld FJM;Bosch BJ;Egberink H
严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）可感染许多动物物种，包括水貂、猫和狗。为了深入了解猫和狗的SARS-CoV-2感染，我们开发并验证了一套血清学检测方法，包括ELISA和病毒中和。对动物感染冠状病毒疾病前的样本和SARS-CoV-2阳性水貂养殖场流浪猫的样本进行评估，证实了这些检测方法适用于特异性抗体检测。此外，我们的研究结果排除了SARS-CoV-2核衣壳蛋白作为抗原的猫和狗样本的血清学筛查。我们分析了2020年4月至5月期间荷兰家猫和家犬的500份血清样本。我们发现0.4%的猫和0.2%的狗血清阳性。虽然在第一波冠状病毒疾病中，接触过未知SARS-CoV-2的猫和狗的血清阳性率较低，但我们的数据强调需要在这两种动物中开展SARS-CoV-2的连续血清监测。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats and dogs, we developed and validated a set of serologic assays, including ELISA and virus neutralization. Evaluation of samples from animals before they acquired coronavirus disease and samples from cats roaming SARS-CoV-2–positive mink farms confirmed the suitability of these assays for specific antibody detection. Furthermore, our findings exclude SARS-CoV-2 nucleocapsid protein as an antigen for serologic screening of cat and dog samples. We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April–May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.
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DOI：
10.1126/science.abe5901
发表时间：
2021-01-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Oude Munnink BB;Sikkema RS;Nieuwenhuijse DF;Molenaar RJ;Munger E;Molenkamp R;van der Spek A;Tolsma P;Rietveld A;Brouwer M;Bouwmeester-Vincken N;Harders F;Hakze-van der Honing R;Wegdam-Blans MCA;Bouwstra RJ;GeurtsvanKessel C;van der Eijk AA;Velkers FC;Smit LAM;Stegeman A;van der Poel WHM;Koopmans MPG
通讯作者：
Koopmans MPG
影响因子：
16.6
作者：
Slot E;Hogema BM;Reusken CBEM;Reimerink JH;Molier M;Karregat JHM;IJlst J;Novotný VMJ;van Lier RAW;Zaaijer HL
通讯作者：
Zaaijer HL
影响因子：
4.3
作者：
Sailleau, Corinne;Dumarest, Marine;Le Poder, Sophie
通讯作者：
Le Poder, Sophie
影响因子：
1.2
作者：
HOHDATSU, T;OKADA, S;KOYAMA, H
通讯作者：
KOYAMA, H
影响因子：
11.8
作者：
Barrs VR;Peiris M;Tam KWS;Law PYT;Brackman CJ;To EMW;Yu VYT;Chu DKW;Perera RAPM;Sit THC
通讯作者：
Sit THC