Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery
Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery
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脱水细胞系 Pv11 的转录组分析推断了干燥耐受性和恢复的机制
DOI：
10.1038/s41598-018-36124-6
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发表时间：
2018
影响因子：
4.6
通讯作者：
Funahashi Akira
中科院分区：
文献类型：
--
作者：
Yamada Takahiro G.;Suetsugu Yoshitaka;Deviatiiarov Ruslan;Gusev Oleg;Cornette Richard;Nesmelov Alexander;Hiroi Noriko;Kikawada Takahiro;Funahashi Akira
非洲蠓的幼虫，Polypedilum vanderplanki，可以进入一种称为脱水共生的无代谢状态，以克服致命的干燥压力。Pv 11细胞系来源于P. vanderplankiembryo在脱水前用海藻糖处理时显示出脱水耐受性，并在再水化后恢复增殖。然而，这种脱水耐受性的分子机制仍然未知。在这里，我们进行了高通量CAGE-seq的mRNA和差异表达的基因分析海藻糖处理，干燥，再水化的Pv 11细胞，然后通过基因本体分析的差异表达的基因。我们检测到海藻糖处理后差异表达的基因参与各种应激反应，有害化学物质的解毒，并上调氧化还原的调节。在脱水阶段，L-异戊酰甲基转移酶和热休克蛋白上调，核糖体蛋白下调。在复水过程中差异表达基因的分析支持的概念，同源重组，核苷酸切除修复，和非同源重组参与恢复过程。这项研究提供了初步的见解Pv 11细胞的极端干燥耐受性的分子机制。
The larvae of the African midge,Polypedilum vanderplanki, can enter an ametabolic state called anhydrobiosis to overcome fatal desiccation stress. The Pv11 cell line, derived fromP. vanderplankiembryo, shows desiccation tolerance when treated with trehalose before desiccation and resumes proliferation after rehydration. However, the molecular mechanisms of this desiccation tolerance remain unknown. Here, we performed high-throughput CAGE-seq of mRNA and a differentially expressed gene analysis in trehalose-treated, desiccated, and rehydrated Pv11 cells, followed by gene ontology analysis of the identified differentially expressed genes. We detected differentially expressed genes after trehalose treatment involved in various stress responses, detoxification of harmful chemicals, and regulation of oxidoreduction that were upregulated. In the desiccation phase, L-isoaspartyl methyltransferase and heat shock proteins were upregulated and ribosomal proteins were downregulated. Analysis of differentially expressed genes during rehydration supported the notion that homologous recombination, nucleotide excision repair, and non-homologous recombination were involved in the recovery process. This study provides initial insights into the molecular mechanisms underlying the extreme desiccation tolerance of Pv11 cells.
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影响因子：
2.8
作者：
Watanabe, M;Kikawada, T;Okuda, T
通讯作者：
Okuda, T
影响因子：
2.7
作者：
Watanabe, Kazuyo;Imanishi, Shigeo;Okuda, Takashi
通讯作者：
Okuda, Takashi
影响因子：
14.9
作者：
Marchler-Bauer A;Lu S;Anderson JB;Chitsaz F;Derbyshire MK;DeWeese-Scott C;Fong JH;Geer LY;Geer RC;Gonzales NR;Gwadz M;Hurwitz DI;Jackson JD;Ke Z;Lanczycki CJ;Lu F;Marchler GH;Mullokandov M;Omelchenko MV;Robertson CL;Song JS;Thanki N;Yamashita RA;Zhang D;Zhang N;Zheng C;Bryant SH
通讯作者：
Bryant SH
影响因子：
0.9
作者：
Davis AJ;Chen DJ
通讯作者：
Chen DJ
影响因子：
4.8
作者：
Muzzin, O;Campbell, EA;Severinov, K
通讯作者：
Severinov, K