A group-1 grass pollen allergen influences the outcome of pollen competition in maize.
A group-1 grass pollen allergen influences the outcome of pollen competition in maize.
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DOI：
10.1371/journal.pone.0000154
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发表时间：
2007-01-17
期刊：
影响因子：
3.7
通讯作者：
Stephenson, Andrew G.
中科院分区：
文献类型：
--
作者：
Valdivia, Elene R.;Wu, Yajun;Li, Lian-Chao;Cosgrove, Daniel J.;Stephenson, Andrew G.
全世界有4亿人患有花粉热和季节性哮喘。这些过敏症的主要病原体是花粉特异性蛋白质，称为1组草花粉过敏原。虽然它们的抗原性的细节已经研究了40年，着眼于免疫治疗，但它们在植物中的功能很少引起注意。玉米m 1是一类丰富的草花粉过敏原，由几个基因编码，这些基因使草细胞壁松动，包括玉米柱头和花柱。我们已经研究了转座子插入到这些基因之一（EXPB 1，最丰富的同种型玉米m 1）的玉米m 1蛋白的生产，花粉活力和花粉管生长，在体外和体内的影响。我们还研究了插入突变对花粉竞争能力的影响，通过实验改变花粉负载的大小沉积到柱头上，使用杂合植物的花粉，然后使用PCR筛选后代转座子的存在。我们发现，插入突变降低了玉米花粉中的玉米m 1的水平，但对花粉活力，在体外花粉管生长或后代的比例时，小花粉负荷沉积到柱头上的父系没有影响。然而，当大的花粉负载沉积到柱头上时，转座子突变在后代中的代表性大大不足，这表明这种主要的花粉过敏原对体内的花粉管生长速率有很大的影响，并在决定花粉-花粉竞争进入胚珠的结果中起着重要的作用。我们建议，这个主要的花粉过敏原的非凡丰度（4%的玉米花粉中的可提取蛋白质）是选择的结果，功能主要是在提供不同的访问胚珠的性状。
Worldwide, 400 million people suffer from hay fever and seasonal asthma. The major causative agents of these allergies are pollen specific proteins called the group-1 grass pollen allergens. Although details of their antigenicity have been studied for 40 years with an eye towards immunotherapy, their function in the plant has drawn scant attention. Zea m 1 constitutes a class of abundant grass pollen allergens coded for by several genes that loosen the walls of grass cells, including the maize stigma and style. We have examined the impact of a transposon insertion into one of these genes (EXPB1, the most abundant isoform of Zea m 1) on the production of Zea m 1 protein, pollen viability, and pollen tube growth, both in vitro and in vivo. We also examined the effect of the insertional mutation on the competitive ability of the pollen by experimentally varying the sizes of the pollen load deposited onto stigmas using pollen from heterozygous plants and then screening the progeny for the presence of the transposon using PCR. We found that the insertional mutation reduced the levels of Zea m 1 in maize pollen, but had no effect on pollen viability, in vitro pollen tube growth or the proportion of progeny sired when small pollen loads are deposited onto stigmas. However, when large pollen loads are deposited onto the stigmas, the transposon mutation is vastly underrepresented in the progeny, indicating that this major pollen allergen has a large effect on pollen tube growth rates in vivo, and plays an important role in determining the outcome of the pollen-pollen competition for access to the ovules. We propose that the extraordinary abundance (4% of the extractable protein in maize pollen) of this major pollen allergen is the result of selection for a trait that functions primarily in providing differential access to ovules.
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影响因子：
3.3
作者：
Falque, M;Décousset, L;Murigneux, A
通讯作者：
Murigneux, A
影响因子：
11.6
作者：
BENSEN, RJ;JOHAL, GS;BRIGGS, SP
通讯作者：
BRIGGS, SP
影响因子：
4.8
作者：
Jones, CG;Lawton, JH;Shachak, M
通讯作者：
Shachak, M
DOI：
10.1073/pnas.94.12.6559
发表时间：
1997-06-10
影响因子：
11.1
作者：
Cosgrove, DJ;Bedinger, P;Durachko, DM
通讯作者：
Durachko, DM
影响因子：
3.5
作者：
GRIFFITH, IJ;SMITH, PM;KNOX, RB
通讯作者：
KNOX, RB