Microstimulation of afferents in the sacral dorsal root ganglia can evoke reflex bladder activity.
Microstimulation of afferents in the sacral dorsal root ganglia can evoke reflex bladder activity.
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DOI：
10.1002/nau.22514
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发表时间：
2015-01
影响因子：
2
通讯作者：
Gaunt, Robert A.
中科院分区：
文献类型：
--
作者：
Bruns, Tim M.;Weber, Douglas J.;Gaunt, Robert A.
关键词：
阴部传入纤维可以通过电刺激来激发膀胱反射活动。虽然这种方法显示了恢复膀胱功能的希望，但刺激所需的通路和整合感觉反馈的传入信号仍然具有挑战性。在骶骨背根神经节，盆腔和阴部传入纤维的汇聚为下尿路神经元提供了一个独特的位置。我们在这项研究中的目标是证明在骶骨背根神经节微刺激的潜力，以唤起反射性膀胱反应。将穿透性微电极阵列植入6只麻醉成年雄性猫左侧S1和S2背根节。当膀胱容量保持在低于漏气量的水平时，采用不同的刺激模式进行单通道和多通道刺激。在5只猫中观察到反射性膀胱兴奋，刺激在1 Hz和30-33 Hz的S1或S2背根节，脉冲幅度为10-50µA。相邻的电极经常会引起截然不同的反应。这些结果证明了低电流微刺激招募反射性膀胱反应的潜力。这样的方法可以与膀胱传入的DRG记录相结合，提供闭环式膀胱神经假体。
Pudendal afferent fibers can be excited using electrical stimulation to evoke reflex bladder activity. While this approach shows promise for restoring bladder function, stimulation of desired pathways and integration of afferent signals for sensory feedback remains challenging. At sacral dorsal root ganglia (DRG), the convergence of pelvic and pudendal afferent fibers provides a unique location for access to lower urinary tract neurons. Our goal in this study was to demonstrate the potential of microstimulation in sacral DRG for evoking reflex bladder responses. Penetrating microelectrode arrays were inserted in the left S1 and S2 DRG of six anesthetized adult male cats. While the bladder volume was held at a level below the leak volume, single and multiple channel stimulation was performed using various stimulation patterns. Reflex bladder excitation was observed in five cats, for stimulation in either S1 or S2 DRG at 1 Hz and 30–33 Hz with a pulse amplitude of 10–50 µA. Bladder relaxation was observed during a few trials. Adjacent electrodes frequently elicited very different responses. These results demonstrate the potential of low-current microstimulation to recruit reflexive bladder responses. An approach such as this could be integrated with DRG recordings of bladder afferents to provide a closed-loop bladder neuroprosthesis.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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