Genomic characterization of a wild-bird-origin pigeon paramyxovirus type 1 (PPMV-1) first isolated in the northwest region of China
Genomic characterization of a wild-bird-origin pigeon paramyxovirus type 1 (PPMV-1) first isolated in the northwest region of China
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中国西北地区首次分离的野生鸟类1型鸽副粘病毒（PPMV-1）的基因组特征
DOI：
10.1007/s00705-016-3156-5
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发表时间：
2017-03
影响因子：
2.7
通讯作者：
Yang Zengqi
中科院分区：
文献类型：
--
作者：
Wang Xinglong;Ren Shanhui;Wang Xiangwei;Wang Chong Yang;Fan Mengfei;Jia Yanqing;Gao Xiaolong;Liu Huan;Xiao Sa;Yang Zengqi
1型鸽子副粘病毒（PPMV-1）是鸽子群中的一种地方性传染病，在许多国家给家禽业造成严重的经济损失。PPMV-1分离株，简称PPMV-1/ qc -01/CH/15，于2015年从中国西北地区的一只大斑啄木鸟中分离得到。结果表明，该病毒全基因组长度为15192 nt，基因排列为3′-NP-P-M-F-HN-L-5′。在F和HN蛋白的功能域中发现了几个氨基酸突变。对分离株的致病性指数进行评价，平均死亡时间（MDT）为72 h，脑内致病性指数（ICPI）为0.925，表明该分离株为中源性。测序结果显示，在融合蛋白的裂解位点有一个强毒的新城疫病毒裂解基序112r - r - q - k - r - f117。接种鸽子后的发病率和死亡率分别为70%和50%，而该病毒在鸡中无致病性。在体内诱导鸽子和鸡产生不同的免疫反应，导致HI血清抗体滴度不同。系统发育和进化距离分析表明，该株PPMV-1属VIa II类亚基因型。据我们所知，这是中国首次发现和分析野生鸟类和家鸽群中PPMV-1共传播。研究结果表明，野生鸟类在PPMV-1的传播过程中发挥了重要作用，为进一步了解PPMV-1在中国的分布和演变提供了有益的参考。
Pigeon paramyxovirus type-1(PPMV-1) is an enzootic in pigeon flocks and causes severe economic losses in the poultry industry in many countries. A PPMV-1 isolate, abbreviated as PPMV-1/QL-01/CH/15, was isolated from a great spotted woodpecker in the northwest region of China in 2015. The complete genome was sequenced, and the results showed that the virus genome was 15,192 nt in length, in the gene arrangement 3′-NP-P-M-F-HN-L-5′. Several amino acid mutations were identified in the functional domains of the F and HN proteins. The pathogenicity index of the isolate was evaluated, and the mean death time (MDT) was 72 h and the intracerebral pathogenicity index (ICPI) was 0.925, indicating that this isolate was mesogenic. Sequencing results showed that it had a virulent Newcastle disease virus cleavage motif112R-R-Q-K-R-F117at the fusion protein cleavage site. Morbidity and mortality were 70% and 50%, after inoculation of pigeons, whereas this virus was nonpathogenic in chickens. Different immune responses of pigeons and chickens were inducedin vivo, which led to different HI serum antibody titers. The results of phylogenetic and evolutionary distance analysis showed that this PPMV-1 strain belonged to sub-genotype VIa in class II. To our knowledge, this is the first identification and analysis of PPMV-1 co-circulation among wild birds and domestic pigeon flocks in China. The data from this study highlight the important role of wild birds in the dissemination of PPMV-1 and provide useful references for improving our understanding of the distribution and evolution of PPMV-1 in China.
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影响因子：
3.8
作者：
Dortmans, J. C. F. M.;Rottier, P. J. M.;Peeters, B. P. H.
通讯作者：
Peeters, B. P. H.
中国朱鹮(Nipponia nippon)分离的两种强效新城疫病毒的系统发育和病原学分析
影响因子：
1.6
作者：
Chen, Shengli;Hao, Huafang;Yang, Zengqi
通讯作者：
Yang, Zengqi
影响因子：
2.8
作者：
Ren, Shanhui;Xie, Xiumei;Yang, Zengqi
通讯作者：
Yang, Zengqi
影响因子：
3.8
作者：
STEWARD, M;VIPOND, IB;EMMERSON, PT
通讯作者：
EMMERSON, PT
影响因子：
3.1
作者：
Li, Z;Yu, M;Wang, LF
通讯作者：
Wang, LF