Diversity of Cervical Microbiota in Asymptomatic Chlamydia trachomatis Genital Infection: A Pilot Study.
Diversity of Cervical Microbiota in Asymptomatic Chlamydia trachomatis Genital Infection: A Pilot Study.
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DOI：
10.3389/fcimb.2017.00321
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发表时间：
2017
影响因子：
5.7
通讯作者：
Sessa R
中科院分区：
文献类型：
--
作者：
Filardo S;Di Pietro M;Porpora MG;Recine N;Farcomeni A;Latino MA;Sessa R
由于其发病率不断增加，沙眼衣原体生殖器感染仍然是世界范围内重要的公共卫生问题。沙眼衣原体感染可导致严重的后遗症，如盆腔炎、梗阻性不孕、早产等。最近，有人提出，宫颈阴道微生物群可能是沙眼衣原体感染以及慢性后遗症发生的重要防御因素。因此，研究与衣原体感染相关的微生物特征至关重要。在这里，我们提出了一项初步研究，旨在通过对已测序的 16s rRNA 基因扩增子进行宏基因组分析来表征沙眼衣原体感染阳性育龄妇女的宫颈微生物群。我们研究的主要发现表明，与健康对照相比，无症状沙眼衣原体阳性女性的细菌多样性在香农多样性和香农均匀度方面显着增加（分别为 P = 0.031 和 P = 0.026）。更重要的是，在加权 UniFrac 分析中，沙眼衣原体阳性女性和健康对照的宫颈微生物群显着分为两个簇（P = 0.0027），这表明两组之间的差异完全取决于细菌类群的相对丰度，而不是存在的细菌类群的类型。此外，沙眼衣原体阳性女性的乳酸菌总体呈下降趋势。以及厌氧菌的增加。这些发现是正在进行的更大规模流行病学研究的一部分，该研究将评估宫颈微生物群的不同细菌群落在沙眼衣原体感染中的潜在作用。
Chlamydia trachomatis genital infection continues to be an important public health problem worldwide due to its increasing incidence. C. trachomatis infection can lead to severe sequelae, such as pelvic inflammatory disease, obstructive infertility, and preterm birth. Recently, it has been suggested that the cervico-vaginal microbiota may be an important defense factor toward C. trachomatis infection as well as the development of chronic sequelae. Therefore, the investigation of microbial profiles associated to chlamydial infection is of the utmost importance. Here we present a pilot study aiming to characterize, through the metagenomic analysis of sequenced 16s rRNA gene amplicons, the cervical microbiota from reproductive age women positive to C. trachomatis infection. The main finding of our study showed a marked increase in bacterial diversity in asymptomatic C. trachomatis positive women as compared to healthy controls in terms of Shannon's diversity and Shannon's evenness (P = 0.031 and P = 0.026, respectively). More importantly, the cervical microbiota from C. trachomatis positive women and from healthy controls significantly separated into two clusters in the weighted UniFrac analysis (P = 0.0027), suggesting that differences between the two groups depended entirely on the relative abundance of bacterial taxa rather than on the types of bacterial taxa present. Furthermore, C. trachomatis positive women showed an overall decrease in Lactobacillus spp. and an increase in anaerobes. These findings are part of an ongoing larger epidemiological study that will evaluate the potential role of distinct bacterial communities of the cervical microbiota in C. trachomatis infection.
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影响因子：
3.7
作者：
Dols JA;Molenaar D;van der Helm JJ;Caspers MP;de Kat Angelino-Bart A;Schuren FH;Speksnijder AG;Westerhoff HV;Richardus JH;Boon ME;Reid G;de Vries HJ;Kort R
通讯作者：
Kort R
影响因子：
10.5
作者：
Ma B;Forney LJ;Ravel J
通讯作者：
Ravel J
DOI：
10.1086/657320
发表时间：
2010-12-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Brotman RM;Klebanoff MA;Nansel TR;Yu KF;Andrews WW;Zhang J;Schwebke JR
通讯作者：
Schwebke JR
影响因子：
4.4
作者：
Downey, Autumn S.;Da Silva, Sandra M.;Morrow, Jayne B.
通讯作者：
Morrow, Jayne B.
DOI：
10.1016/j.ejogrb.2005.11.036
发表时间：
2006-11-01
影响因子：
2.6
作者：
Mijac, Vera D.;Dukic, Slobodanka V.;Ranin, Lazar T.
通讯作者：
Ranin, Lazar T.