A plant-unique protein BLISTER coordinates with core retromer to modulate endosomal sorting of plasma membrane and vacuolar proteins.

A plant-unique protein BLISTER coordinates with core retromer to modulate endosomal sorting of plasma membrane and vacuolar proteins.
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植物独特的蛋白质 BLISTER 与核心逆转录酶协调调节质膜和液泡蛋白的内体分选

DOI:
10.1073/pnas.2211258120
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发表时间:
2023-01-03
影响因子:
11.1
通讯作者:
--
中科院分区:
综合性期刊1区
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--
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意义 作为一种重要的膜重塑机制,逆转录酶与内体结合并调节植物中液泡储存蛋白的运输和内吞蛋白的再循环。然而,参与调节植物逆转录酶核心复合物的组装和内体募集的因素仍然难以捉摸。在这里,我们将 BLISTER (BLI) 确定为植物特有的内体调节因子,它影响核心逆转录酶复合物的组装和膜募集,从而控制可溶性液泡蛋白的液泡运输和内吞质膜蛋白的再循环。这项研究拓宽了我们对植物中逆转录酶调节机制的理解,并强调了植物特异性蛋白 BLI 在调节逆转录酶调节的内体运输方面的独特特性。
Significance As an important membrane remodeling machinery, the retromer binds to endosomes and regulates the trafficking of vacuolar storage proteins and the recycling of endocytosed proteins in plants. However, the factors involved in regulating the assembly and endosomal recruitment of the plant retromer core complex remain elusive. Here, we identified BLISTER (BLI) as a plant-unique endosomal regulator, which affects the assembly and membrane recruitment of the core retromer complex, thereby controlling the vacuolar transport of soluble vacuolar proteins and the recycling of endocytosed plasma membrane proteins. This study broadens our understandings of the mechanism underlying retromer regulation in plants and highlights the unique properties of the plant-specific protein BLI in modulating retromer-regulated endosomal trafficking.
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