Labile catalytic packaging of DNA/siRNA: control of gold nanoparticles "out" of DNA/siRNA complexes.
Labile catalytic packaging of DNA/siRNA: control of gold nanoparticles "out" of DNA/siRNA complexes.
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DOI：
10.1021/nn901796n
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发表时间：
2010-07-27
期刊：
影响因子：
17.1
通讯作者：
He, Huixin
中科院分区：
文献类型：
--
作者：
Chen, Alex M.;Taratula, Oleh;Wei, Dongguang;Yen, Hsin-I;Thomas, Thresia;Thomas, T. J.;Minko, Tamara;He, Huixin
关键词：
开发了一种新的方法，通过使用Au纳米颗粒作为“不稳定的催化”包装剂，有效地包装和递送核酸与低代聚丙烯亚胺（PPI）树枝状聚合物。Au纳米颗粒（Au NPs）帮助低代树枝状聚合物将核酸包装成离散的纳米颗粒，但不包括在最终的DNA/siRNA复合物中。因此，通过在将Au NP递送至靶细胞之前从所得核酸复合物中选择性地除去所述Au NP，消除与所述NP相关的潜在毒性问题成为可能。这是在核酸包装和递送应用中使用无机工程纳米颗粒的新概念。此外，与仅通过低代树枝状聚合物（第3代）制造的siRNA纳米结构（主要是随机聚集的纳米纤维）相比，使用这种新方法（通过用G3 PPI修饰的Au NPs）包装的siRNA纳米颗粒可以被癌细胞内化，并且递送的siRNA可以有效地沉默其靶mRNA。这种新方法的mRNA沉默效率甚至上级更高代的树枝状聚合物（第5代）。
A novel approach was developed to efficiently package and deliver nucleic acids with low generation polypropylenimine (PPI) dendrimers by using Au nanoparticles as a “labile catalytic” packaging agent. The Au nanoparticles (Au NPs) helped low generation dendrimers to package nucleic acids into discrete nanoparticles but are not included in the final DNA/siRNA complexes. Therefore it becomes possible to eliminate the potential toxic problems associated with Au NPs by selectively removing the Au NPs from the resulting nucleic acid complexes before their delivery to targeted cells. This is a new concept in using inorganic engineered nanoparticles in nucleic acid packaging and delivery applications. Furthermore, compared to the siRNA nanostructures (mainly randomly aggregated nanofibers) fabricated by low generation dendrimer alone (Generation 3), the siRNA nanoparticles packaged using this novel approach (by Au NPs modified with G3 PPI) can be internalized by cancer cells and the delivered siRNAs can efficiently silence their target mRNA. The efficiency of mRNA silencing by this novel approach is even superior to higher generation dendrimers (Generation 5).
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