Resistome and Genome Analysis of an Extensively Drug-Resistant Klebsiella michiganensis KMIB106: Characterization of a Novel KPC Plasmid pB106-1 and a Novel Cointegrate Plasmid pB106-IMP Harboring bla(IMP-4) and bla(SHV-12).
Resistome and Genome Analysis of an Extensively Drug-Resistant Klebsiella michiganensis KMIB106: Characterization of a Novel KPC Plasmid pB106-1 and a Novel Cointegrate Plasmid pB106-IMP Harboring bla(IMP-4) and bla(SHV-12).
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DOI：
10.3390/antibiotics12091463
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发表时间：
2023-09-20
影响因子：
4.8
通讯作者：
Chen, Dingqiang
中科院分区：
文献类型：
--
作者：
Wang, Linjing;Chen, Haijun;Liu, Wanting;Yang, Ling;Xu, Zhenbo;Chen, Dingqiang
密歇根克雷伯氏菌是一种新近出现的引起医院感染的人类病原体。本研究旨在对一株广泛耐药的密歇根克雷伯氏菌临床株KMIB106进行全基因组序列分析。使用PacBio RS III系统和Illumina Nextseq 500对该菌株的全基因组进行了测序。分别用RAST、PROKKA和质粒搜索器进行注释分析、转座元件分析和抗性基因鉴定。结果表明，KMIB106对包括碳青霉烯类在内的多种抗菌药物具有耐药性，但对氨曲南仍敏感。KMIB106的基因组由1条染色体和3个预测的质粒组成。重要的是，一个新的KPC质粒pB106-1在其可变区中以非常不同的顺序携带了一系列抗性基因，包括mphA、MSRE、mphE、arr-3、addA16、sul1、dfrA27、etD和fosA3。PB106-2是一种典型的无抗性基因的IncFII质粒。PB106-IMP由IncN和IncX3两个骨架组成，鉴定出两个抗性基因blaIMP-4和blaSHV-12。本研究首次报道了从南方中国地区呼吸道感染患儿体内分离到的一株广泛耐药的密歇根克雷伯氏菌，该菌株携带3个百万质粒，其中pB106-1首次被鉴定为以不同顺序携带一系列耐药基因，pB106-IMP被鉴定为一种新的IncN-IncX3共整合质粒，含有blaIMP-4和blaSHV-12两个耐药基因。
Klebsiella michiganensis is a recently emerging human pathogen causing nosocomial infections. This study aimed to characterize the complete genome sequence of a clinical Klebsiella michiganensis strain KMIB106 which exhibited extensive drug-resistance. The whole genome of the strain was sequenced using PacBio RS III systems and Illumina Nextseq 500. Annotation, transposable elements and resistance gene identification were analyzed by RAST, prokka and Plasmid Finder, respectively. According to the results, KMIB106 was resistant to multiple antimicrobials, including carbapenems, but it remained susceptible to aztreonam. The genome of KMIB106 consisted of a single chromosome and three predicted plasmids. Importantly, a novel KPC plasmid pB106-1 was found to carry the array of resistance genes in a highly different order in its variable regions, including mphA, msrE, mphE, ARR-3, addA16, sul1, dfrA27, tetD and fosA3. Plasmid pB106-2 is a typical IncFII plasmid with no resistant gene. Plasmid pB106-IMP consists of the IncN and IncX3 backbones, and two resistance genes, blaIMP-4 and blaSHV-12, were identified. Our study for the first time reported an extensively drug-resistant Klebsiella michiganensis strain recovered from a child with a respiratory infection in Southern China, which carries three mega plasmids, with pB106-1 firstly identified to carry an array of resistance genes in a distinctive order, and pB106-IMP identified as a novel IncN-IncX3 cointegrate plasmid harboring two resistance genes blaIMP-4 and blaSHV-12.
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Pourcel, Christine