Diurnal and light regulation of sulphur assimilation and glucosinolate biosynthesis in Arabidopsis.
Diurnal and light regulation of sulphur assimilation and glucosinolate biosynthesis in Arabidopsis.
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DOI：
10.1093/jxb/ers378
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发表时间：
2013-02
影响因子：
6.9
通讯作者：
Kopriva S
中科院分区：
文献类型：
--
作者：
Huseby S;Koprivova A;Lee BR;Saha S;Mithen R;Wold AB;Bengtsson GB;Kopriva S
硫代葡萄糖苷是一类重要的含硫次生代谢产物，参与植物对病原菌的防御。近年来，硫代葡萄糖苷生物合成和硫酸盐同化之间的许多调节联系被建立起来。由于硫酸盐同化经历昼夜节律和光调节，这项研究分析是否同样是如此的硫代葡萄糖苷的生物合成。硫代葡萄糖苷和谷胱甘肽的水平被发现是在白天比在夜间更高。这与硫酸盐吸收的变化以及硫酸盐同化途径的关键酶腺苷5 '-磷酸硫酸还原酶的活性一致。相应地，通过硫酸盐同化的通量在白天比在夜间高，通过初级同化的最大通量之前最大掺入硫代葡萄糖苷。长时间的黑暗导致硫代葡萄糖苷含量的强烈减少。这种黑暗适应植物的再照明诱导硫代葡萄糖苷生物合成的许多基因的mRNA的积累，导致增加硫代葡萄糖苷的生物合成。硫代葡萄糖苷合成基因以及许多初级硫酸盐同化基因的光调节至少部分地由LONG HYPOCOTYL 5（HY 5）转录调节子控制。因此，硫代葡萄糖苷的生物合成与硫酸盐同化高度共调节。
Glucosinolates are a major class of sulphur-containing secondary metabolites involved in plant defence against pathogens. Recently many regulatory links between glucosinolate biosynthesis and sulphate assimilation were established. Since sulphate assimilation undergoes diurnal rhythm and is light regulated, this study analysed whether the same is true for glucosinolate biosynthesis. The levels of glucosinolates and glutathione were found to be higher during the day than during the night. This agreed with variation in sulphate uptake as well as activity of the key enzyme of the sulphate assimilation pathway, adenosine 5’-phosphosulphate reductase. Correspondingly, the flux through sulphate assimilation was higher during the day than during the night, with the maximum flux through primary assimilation preceding maximal incorporation into glucosinolates. Prolonged darkness resulted in a strong reduction in glucosinolate content. Re-illumination of such dark-adapted plants induced accumulation of mRNA for many genes of glucosinolate biosynthesis, leading to increased glucosinolate biosynthesis. The light regulation of the glucosinolate synthesis genes as well as many genes of primary sulphate assimilation was controlled at least partly by the LONG HYPOCOTYL5 (HY5) transcription regulator. Thus, glucosinolate biosynthesis is highly co-regulated with sulphate assimilation.
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影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
影响因子：
6.9
作者：
Kusnierczyk, Anna;Winge, Per;Bones, Atle Magnar
通讯作者：
Bones, Atle Magnar
影响因子：
3.7
作者：
Kim, Sang-Gyu;Yon, Felipe;Baldwin, Ian T.
通讯作者：
Baldwin, Ian T.
影响因子：
9.4
作者：
Gigolashvili, Tamara;Engqvist, Martin;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
7.4
作者：
Celenza, JL;Quiel, JA;Bender, J
通讯作者：
Bender, J