Treatment of HIV tissue culture infection with monoclonal antibody-ricin A chain conjugates.
Treatment of HIV tissue culture infection with monoclonal antibody-ricin A chain conjugates.
复制标题
用单克隆抗体-蓖麻毒素 A 链缀合物治疗 HIV 组织培养感染。
作者：
S. Pincus;K. Wehrly;B. Chesebro
mAb 907是针对HIV的包膜蛋白。该抗体识别的抗原表位在感染LAV/HTLV-IIIB病毒株的组织培养细胞表面以中等密度表达。我们将抗体907偶联到蓖麻毒素A链（RAC）上。抗体- rac偶联物抑制hiv感染细胞的蛋白质合成和细胞生长。与RAC偶联的无关抗体没有作用。最重要的是，通过在易感单层细胞上产生病毒病灶来测量，用该偶联物处理感染细胞可显著抑制感染性病毒的产生。将hiv感染的靶细胞暴露于偶联物短至1小时，可导致细胞死亡。艾滋病患者的血清抑制了907-RAC偶联物的毒性，但没有完全抑制。第二种抗体，被称为BM-1，它识别病毒感染细胞表面的碳水化合物Ag，与RAC结合。BM-1-RAC偶联物不能杀死hiv感染的细胞，突出了靶Ag的重要性。在hiv感染细胞表面识别Ag的抗体产生的免疫毒素可能在艾滋病的治疗中具有实用价值。
mAb 907 is directed against the envelope protein of the HIV. The epitope recognized by this antibody is expressed in moderate density on the surface of tissue culture cells infected with the LAV/HTLV-IIIB strain of HIV. We have coupled antibody 907 to ricin A chain (RAC). The antibody-RAC conjugate inhibited protein synthesis and cell growth in HIV-infected cells. An irrelevant antibody conjugated to RAC had no effect. Most important, treatment of infected cells with the conjugate markedly inhibited the production of infectious virus, as measured by the production of viral foci on susceptible monolayer cells. Exposure of HIV-infected target cells to the conjugate for as short a period as 1 h resulted in cell death. Serum of AIDS patients inhibited, but did not completely suppress, the toxicity of the 907-RAC conjugate. A second antibody, designated BM-1, which recognizes a carbohydrate Ag on the surface of virally infected cells, was conjugated to RAC. The BM-1-RAC conjugate did not kill HIV-infected cells, highlighting the importance of the target Ag. Immunotoxins produced with antibodies that recognize Ag on the surface of HIV-infected cells may have utility in the therapy of AIDS.
影响因子：
56.9
作者：
E. Vitetta;R. Fulton;R. May;M. Till;J. Uhr
通讯作者：
E. Vitetta;R. Fulton;R. May;M. Till;J. Uhr
固定化蛋白A灌注血浆对乳腺癌的影响的初步观察。
DOI：
10.1056/nejm198111123052006
发表时间：
1981
期刊：
The New England journal of medicine
影响因子：
--
作者：
Terman,DS;Young,JB;Shearer,WT;Ayus,C;Lehane,D;Mattioli,C;Espada,R;Howell,JF;Yamamoto,T;Zaleski,HI;Miller,L;Frommer,P;Feldman,L;Henry,JF;Tillquist,R;Cook,G;Daskal,Y
通讯作者：
Daskal,Y
HIV 感染的细胞被 rCD4-蓖麻毒素 A 链杀死。
DOI：
10.1126/science.2847316
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Till,MA;Ghetie,V;Gregory,T;Patzer,EJ;Porter,JP;Uhr,JW;Capon,DJ;Vitetta,ES
通讯作者：
Vitetta,ES