Multi-dimensional liquid chromatography in proteomics--a review.
Multi-dimensional liquid chromatography in proteomics--a review.
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DOI：
10.1016/j.aca.2010.02.001
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发表时间：
2010-04-07
影响因子：
6.2
通讯作者：
Buck C
中科院分区：
文献类型：
--
作者：
Zhang X;Fang A;Riley CP;Wang M;Regnier FE;Buck C
蛋白质组学是对蛋白质，特别是其表达，结构和功能的大规模研究。这种新兴的技术组合旨在描述和表征生物系统中所有表达的蛋白质。由于质谱仪的质量检测上限，蛋白质通常被消化成肽，然后从这种复杂的酶消化物中分离、鉴定和定量肽。首先消化蛋白质，然后通过质谱分析肽裂解片段的问题是产生大量肽，其压倒直接质谱分析。蛋白质组学的液相色谱方法的目的是将肽混合物破碎，以通过质谱法最大限度地鉴定和定量组分肽。本综述将重点介绍现有的多维液相色谱（MDLC）平台开发的蛋白质组学和它们的应用与其他技术，如稳定同位素标记相结合。我们还对未来可能的发展提出了一些看法。
Proteomics is the large-scale study of proteins, particularly their expression, structures and functions. This still-emerging combination of technologies aims to describe and characterize all expressed proteins in a biological system. Because of upper limits on mass detection of mass spectrometers, proteins are usually digested into peptides and the peptides are then separated, identified and quantified from this complex enzymatic digest. The problem in digesting proteins first and then analyzing the peptide cleavage fragments by mass spectrometry is that huge numbers of peptides are generated that overwhelm direct mass spectral analyses. The objective in the liquid chromatography approach to proteomics is to fractionate peptide mixtures to enable and maximize identification and quantification of the component peptides by mass spectrometry. This review will focus on existing multidimensional liquid chromatographic (MDLC) platforms developed for proteomics and their application in combination with other techniques such as stable isotope labeling. We also provide some perspectives on likely future developments.
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DOI：
10.1016/s0021-9673(00)96972-3
发表时间：
1990-01-19
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALPERT, AJ
通讯作者：
ALPERT, AJ
影响因子：
4.4
作者：
Bouchal, Pavel;Roumeliotis, Theodoros;Garbis, Spiros D.
通讯作者：
Garbis, Spiros D.
影响因子：
17.3
作者：
Blackstock, WP;Weir, MP
通讯作者：
Weir, MP
影响因子：
3.4
作者：
Asara, John M.;Christofk, Heather R.;Cantley, Lewis C.
通讯作者：
Cantley, Lewis C.
影响因子：
46.9
作者：
Blagoev, B;Ong, SE;Mann, M
通讯作者：
Mann, M