The Change of Left Ventricular Function in Rats with Subclinical Hypothyroid and the Effects of Thyroxine Replacement.
The Change of Left Ventricular Function in Rats with Subclinical Hypothyroid and the Effects of Thyroxine Replacement.
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亚临床甲状腺功能减退大鼠左心室功能的变化及甲状腺素替代的影响
DOI：
10.1155/2018/8682765
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发表时间：
2018
影响因子：
2.8
通讯作者：
Tian L
中科院分区：
文献类型：
--
作者：
Chen X;Gao C;Gong N;Wang Y;Tian L
目的探讨不同TSH水平亚临床甲状腺功能减退症患者血清Serca 2a、Ryr 2、脂肪因子与左心功能的关系及L-T4治疗对这些指标的影响。方法雄性Wistar大鼠65只，随机分为对照组，sHT A、B、C组，sHT + T4组。用他巴唑（MMI）诱导sHT大鼠，MMI给药8周后给予sHT + T4大鼠L-T4治疗。采用放射免疫法和酶联免疫吸附法检测血清TT 4、TSH、APN、chemerin、TNF-α水平，经锁骨下动脉插管采用PowerLab系统检测左室功能。RT-PCR、Western blot和免疫组化检测Serca 2a、Ryr 2、APN、chemerin和TNF-α的表达。结果sHT组血清TSH、chemerin、TNF-α水平显著升高，Serca 2a、Ryr 2、APN水平显著降低。sHT + T4组左心室压力和心率均显著低于对照组和sHT + T4组。组织学检查显示sHT大鼠心脏病理改变。L-T4治疗可降低TSH水平，改善左心室功能。结论TSH可通过调节多种因素损害sHT大鼠左室功能，L-T4治疗可改善sHT大鼠左室功能。
Objective The main purpose of this study was to explore the relationships between serca2a, Ryr2, adipokines, and the left ventricular function in the subclinical hypothyroidism with different TSH levels and to determine the impact of L-T4 treatment on these indexes. Methods Sixty-five male Wistar rats were randomly divided into five groups: control group; sHT A, B, and C group; and sHT + T4 group. The sHT rats were induced by methimazole (MMI), and the sHT + T4 rats were administered with L-T4 treatment after 8 weeks of MMI administration. Serum TT4, TSH, APN, chemerin, and TNF-α were detected by radioimmunoassay kits and ELISA kits; left ventricular function was measured by PowerLab system via subclavian artery catheter. The expression of Serca2a, Ryr2, APN, chemerin, and TNF-α were detected by RT-PCR, Western blot, and immunohistochemistry. Results The sHT groups had significantly higher TSH, chemerin, and TNF-α and lower Serca2a, Ryr2, and APN. The left ventricular pressure and heart rate in sHT groups were significantly lower in control and sHT + T4 group. Histopathological examination revealed the pathological changes in the sHT rats' heart. L-T4 administration reduced TSH level and improved left ventricular function. Conclusions TSH can impair left ventricular function by regulating several factors, and L-T4 treatment ameliorates it in sHT rats.
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影响因子：
5.4
作者：
Shatynska-Mytsyk, I.;Rodrigo, L.;Kruzliak, P.
通讯作者：
Kruzliak, P.
影响因子：
29
作者：
Gorski PA;Ceholski DK;Hajjar RJ
通讯作者：
Hajjar RJ
影响因子：
3.7
作者：
Leal, Anna Lucia R. C.;Pantaleao, Thiago U.;da Costa, Vania Maria Correa
通讯作者：
da Costa, Vania Maria Correa
影响因子：
1.8
作者：
Santi, A.;Baldissareli, J.;Loro, V. L.
通讯作者：
Loro, V. L.
影响因子：
3.4
作者：
Seifi, Samira;Tabandeh, Mohammad Reza;Sarkoohi, Parisa
通讯作者：
Sarkoohi, Parisa