A novel biosensor for ultrasensitive detection of fungal genes.
A novel biosensor for ultrasensitive detection of fungal genes.
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DOI：
10.1016/j.bios.2022.114986
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发表时间：
2023-02-15
影响因子：
12.6
通讯作者：
中科院分区：
文献类型：
--
作者：
真菌感染是一个迅速增加的公共卫生问题，由于其高发病率和死亡率，特别是在免疫系统受损的人群中。因此，这些疾病的快速和准确的诊断是必要的，以改善患者的预后。不幸的是，目前的临床化学实践依赖于冗长的培养方法，不足以满足快速周转的要求。在这里，我们提出了一种具有成本效益和强大的核酸传感器，可以识别组织胞浆菌病引起的真菌基因的存在，在全血或支气管肺泡灌洗（BAL）样本，远远早于目前的方法。我们的新的检测方法涉及杂交的目标基因序列与固定的核酸探针，允许直接，无标记检测Hcp 100，CBP 1，和M抗原基因通过电化学分析。产生的电流归因于样品溶液中存在真菌靶标。该检测试剂盒可对DNA分子进行超灵敏检测，检测限（LOD）值低至100 aM，足以满足临床诊断需求。此外，与当前临床程序的3-4周周转时间相比，样本到结果的周转时间不到90分钟。
Fungal infections are a rapidly increasing public health problem due to their high morbidity and mortality rates, especially in populations with compromised immune systems. Rapid and accurate diagnosis of these diseases is, therefore, necessary to improve the prognosis of afflicted patients. Unfortunately, current clinical chemistry practice relies on lengthy culturing methods that are insufficient to meet the fast turnaround requirements. Here we present a cost-effective and robust nucleic acid sensor that can identify the presence of histoplasmosis causing fungal genes, in whole blood or Bronchoalveolar lavage (BAL) samples, far earlier than current methods. Our novel assay involves the hybridization of target gene sequences with immobilized nucleic acid probes, allowing direct, label-free detection of Hcp100, CBP1, and M antigen genes through electrochemical analysis. The resultant current is attributed to the presence of fungal targets in the sample solution. The assay provides ultra-sensitive detection of DNA molecules with a limit of detection (LOD) values down to 100 aM, sufficient to meet the clinical diagnostic need. In addition, the turnaround time for the sample to result is less than 90 minutes compared to the current clinical procedure’s turnaround time of 3–4 weeks.
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影响因子：
12.6
作者：
Desai, Dignya;Kumar, Ashok;Datta, Manali
通讯作者：
Datta, Manali
影响因子：
13.6
作者：
Ostrov N;Jimenez M;Billerbeck S;Brisbois J;Matragrano J;Ager A;Cornish VW
通讯作者：
Cornish VW
影响因子：
5.4
作者：
Kozel, Thomas R.;Wickes, Brian
通讯作者：
Wickes, Brian
影响因子：
12.6
作者：
Tichoniuk, M.;Gwiazdowska, D.;Filipiak, M.
通讯作者：
Filipiak, M.
影响因子：
11.8
作者：
Kami, M;Fukui, T;Hirai, H
通讯作者：
Hirai, H