Whole-mount in situ hybridization reveals the expression of the Xl-Fli gene in several lineages of migrating cells in Xenopus embryos.
Whole-mount in situ hybridization reveals the expression of the Xl-Fli gene in several lineages of migrating cells in Xenopus embryos.
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整体原位杂交揭示了 Xl-Fli 基因在非洲爪蟾胚胎迁移细胞的几个谱系中的表达。
DOI：
10.1387/ijdb.8901193
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
P. Remy
中科院分区：
文献类型：
--
作者：
D. Meyer;P. Stiegler;C. Hindelang;A. Mager;P. Remy
采用整体原位杂交技术研究了非洲爪蟾胚胎发生过程中ets家族转录因子Xl-Fli基因的表达。地高辛标记的反义RNA探针通过体外转录合成并用于杂交反应。除了先前报道的在被神经嵴细胞侵入的区域中的表达（Meyer et al.，1993），我们观察到Xl-Fli基因在许多受重要细胞迁移和/或上皮<==>间充质转变影响的区域中表达：在心脏的内皮细胞中、在血管中、沿着原肾管迁移途径和在垂体水平。FLI蛋白参与表达的指导线索和/或修改的细胞粘附性能的可能性进行了讨论。筛选的启动子库与共识序列，结合的FLI蛋白具有高亲和力，揭示了推定的FLI反应元件的存在下，在许多基因编码的粘附分子或组分的细胞外基质。
The expression of the Xl-Fli gene, which belongs to the ets family of transcription factors, was studied by whole-mount in situ hybridization during Xenopus embryogenesis. Digoxigenin-labeled antisense RNA probes were synthesized by in vitro transcription and used in the hybridization reaction. In addition to expression in territories invaded by neural crest cells reported earlier (Meyer et al., 1993), we observed Xl-Fli gene expression in a number of regions affected by important cellular migrations and/or epithelium<==>mesenchyme transitions: in the endothelial cells of the heart, in blood vessels, along the pronephric duct migration pathway and at the level of hypophysis. The possibility that the FLI protein is involved in the expression of guidance cues and/or modification of the cellular adhesion properties is discussed. A screening of a promoter library with a consensus sequence, bound by the FLI protein with a high affinity, revealed the presence of putative FLI response elements in a number of genes encoding adhesion molecules or components of the extra-cellular matrix.
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DOI：
10.1073/pnas.87.17.6497
发表时间：
1990
影响因子：
11.1
作者：
Jones,FS;Crossin,KL;Cunningham,BA;Edelman,GM
通讯作者：
Edelman,GM
三个迁移两栖动物胚胎细胞群体利用不同的引导信号。
影响因子：
2.7
作者：
Thibaudeau,G;Drawbridge,J;Dollarhide,AW;Haque,T;Steinberg,MS
通讯作者：
Steinberg,MS
影响因子：
4.6
作者：
Andres Collazo;M. Bronner‐Fraser;Scott E. Fraser
通讯作者：
Andres Collazo;M. Bronner‐Fraser;Scott E. Fraser
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC
影响因子：
4.6
作者：
C. Kelley;Heather Blumberg;Leonard I. Zon;Todd Evans
通讯作者：
C. Kelley;Heather Blumberg;Leonard I. Zon;Todd Evans