Intercellular adhesion and biocide resistance in nontypeable Haemophilus influenzae biofilms.
Intercellular adhesion and biocide resistance in nontypeable Haemophilus influenzae biofilms.
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DOI：
10.1016/j.micpath.2009.01.004
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发表时间：
2009-04
影响因子：
3.8
通讯作者：
Kaplan JB
中科院分区：
文献类型：
--
作者：
Izano EA;Shah SM;Kaplan JB
由不可分型的流感嗜血杆菌（NTHi）引起的呼吸道感染是一个主要的医学问题。有证据表明，在粘膜表面上形成生物膜的能力可能在NTHi发病机制中起作用。然而，有助于NTHi生物膜凝聚力的因素在很大程度上仍然未知。在这项研究中，我们调查了生物膜的生长和脱离表型的八个NTHi临床菌株在体外。我们发现大多数菌株在试管中静态培养时在6小时内产生生物膜。培养基中添加蛋白酶K或DNA酶I可抑制生物膜的形成。两种酶也引起预形成的NTHi生物膜的显著脱离。这些发现表明，蛋白质粘附素和细胞外DNA有助于NTHi生物膜的凝聚力。用DNA酶I处理在离心过滤装置中培养的NTHi生物膜，而不是用蛋白酶K处理，导致通过生物膜的流体对流显著减少。这些结果表明，细胞外DNA是NTHi生物膜基质的主要体积组分。在微量滴定板中培养的NTHi生物膜的机械或酶促破坏显著增加了它们对SDS、氯化十六烷基吡啶、葡萄糖酸氯己定、聚维酮碘和次氯酸钠杀死的敏感性。这些发现表明，NTHi生物膜中的杀生物剂抗性在很大程度上是由生物膜基质的粘附性和保护性介导的。了解NTHi生物膜中生物膜凝聚力和杀生物剂抗性的机制可能会导致治疗NTHi相关感染的新方法。
Respiratory infections caused by nontypeable Haemophilus influenzae (NTHi) are a major medical problem. Evidence suggests that the ability to form biofilms on mucosal surfaces may play a role in NTHi pathogenesis. However, the factors that contribute to NTHi biofilm cohesion remain largely unknown. In this study we investigated the biofilm growth and detachment phenotypes of eight NTHi clinical strains in vitro. We found that the majority of strains produced biofilms within 6 hours when cultured statically in tubes. Biofilm formation was inhibited when culture medium was supplemented with proteinase K or DNase I. Both enzymes also caused significant detachment of pre-formed NTHi biofilms. These findings indicate that both proteinaceous adhesins and extracellular DNA contribute to NTHi biofilm cohesion. Treatment of NTHi biofilms cultured in centrifugal filter devices with DNase I, but not with proteinase K, caused a significant decrease in fluid convection through the biofilms. These results suggest that extracellular DNA is the major volumetric component of the NTHi biofilm matrix. Mechanical or enzymatic disruption of NTHi biofilms cultured in microtiter plates significantly increased their sensitivity to killing by SDS, cetylpyridinium chloride, chlorhexidine gluconate, povidone iodine and sodium hypochlorite. These findings indicate that biocide resistance in NTHi biofilms is mediated to a large part by the cohesive and protective properties of the biofilm matrix. Understanding the mechanisms of biofilm cohesion and biocide resistance in NTHi biofilms may lead to new methods for treating NTHi-associated infections.
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影响因子：
3.2
作者：
Jurcisek, Joseph A.;Bakaletz, Lauren O.
通讯作者：
Bakaletz, Lauren O.
影响因子：
3.1
作者：
Leroy, Magali;Cabral, Howard;Goldstein, Richard
通讯作者：
Goldstein, Richard
影响因子：
7.6
作者：
Izano, E. A.;Wang, H.;Kaplan, J. B.
通讯作者：
Kaplan, J. B.
DOI：
10.1073/pnas.75.3.1534
发表时间：
1978-01-01
影响因子：
11.1
作者：
MOXON, ER;MURPHY, PA
通讯作者：
MURPHY, PA
影响因子：
3.2
作者：
Kaplan, JB;Velliyagounder, K;Ramasubbu, N
通讯作者：
Ramasubbu, N