Graphene and nanowire transistors for cellular interfaces and electrical recording.
Graphene and nanowire transistors for cellular interfaces and electrical recording.
复制标题
DOI：
10.1021/nl1002608
复制
发表时间：
2010-03-10
期刊：
影响因子：
10.8
通讯作者：
Lieber CM
中科院分区：
文献类型：
--
作者：
Cohen-Karni T;Qing Q;Li Q;Fang Y;Lieber CM
纳米线场效应晶体管（NW-FET）由于其优异的灵敏度和与细胞膜形成强耦合界面的能力而被证明是用于与细胞和组织的纳米级生物电子界面的强大构建块。石墨烯也被证明是纳米级电子器件的有吸引力的构建块，尽管对其与细胞和组织的界面知之甚少。在这里，我们报告了石墨烯场效应晶体管（Gra-FET）以及与生电细胞连接的组合Gra和NW-FET的第一项研究。从自发跳动的鸡胚心肌细胞记录的Gra-FET电导信号产生了明确的细胞外信号，信噪比通常>4。电导信号幅度通过改变Gra-FET的工作区域，通过改变水门电位，Vwg进行调谐。对于不同的Vwg，从心肌细胞记录的信号导致恒定的校准的细胞外电压，表明稳健的石墨烯/细胞界面。值得注意的是，Vwg在狄拉克点上的变化证明了预期的信号极性翻转，从而首次允许简单地通过偏移Vwg从相同的Gra-FET实现n型和p型记录。此外，作为Gra-FET器件尺寸的函数和与NW-FET的峰-峰记录信号宽度的比较允许评估细胞外记录中的相对分辨率。具体而言，峰-峰宽度随着Gra-FET器件的面积而增加，表明来自搏动细胞外膜上不同点的平均信号。与二维Gra-FET器件并排合并的一维硅NW-FET进一步突出了时间分辨率和来自不同类型器件的相同单元的多路复用测量的限制。Gra和NW-FET的独特和互补功能可以在未来的生物电子领域开辟独特的机会。
Nanowire field-effect transistors (NW-FETs) have been shown to be powerful building blocks for nanoscale bioelectronic interfaces with cells and tissue due to their excellent sensitivity and their capability to form strongly coupled interfaces with cell membranes. Graphene has also been shown to be an attractive building block for nanoscale electronic devices, although little is known about its interfaces with cells and tissue. Here we report the first studies of graphene field effect transistors (Gra-FETs) as well as combined Gra- and NW-FETs interfaced to electrogenic cells. Gra-FET conductance signals recorded from spontaneously beating embryonic chicken cardiomyocytes yield well-defined extracellular signals with signal-to-noise ratio routinely >4. The conductance signal amplitude was tuned by varying the Gra-FET working region through changes in water gate potential, Vwg. Signals recorded from cardiomyocytes for different Vwg result in constant calibrated extracellular voltage, indicating a robust graphene/cell interface. Significantly, variations in Vwg across the Dirac point demonstrate the expected signal polarity flip, thus allowing, for the first time, both n- and p-type recording to be achieved from the same Gra-FET simply by offsetting Vwg. In addition, comparisons of peak-to-peak recorded signal widths made as a function of Gra-FET device sizes and versus NW-FETs allowed an assessment of relative resolution in extracellular recording. Specifically, peak-to-peak widths increased with the area of Gra-FET devices, indicating an averaged signal from different points across the outer membrane of the beating cells. One-dimensional silicon NW- FETs incorporated side by side with the two-dimensional Gra-FET devices further highlighted limits in both temporal resolution and multiplexed measurements from the same cell for the different types of devices. The distinct and complementary capabilities of Gra- and NW-FETs could open up unique opportunities in the field of bioelectronics in the future.
登录
查看更多内容
影响因子：
12.6
作者：
Heer, F.;Hafizovic, S.;Hierlemann, A.
通讯作者：
Hierlemann, A.
影响因子：
10.8
作者：
Park, Jung;Bauer, Sebastian;Schmuki, Patrik
通讯作者：
Schmuki, Patrik
影响因子：
2.9
作者：
Arnold, M;Cavalcanti-Adam, EA;Spatz, JP
通讯作者：
Spatz, JP
影响因子：
14.8
作者：
Patolsky, Fernando;Zheng, Gengfeng;Lieber, Charles M.
通讯作者：
Lieber, Charles M.
影响因子：
4
作者：
Blake, P.;Hill, E. W.;Geim, A. K.
通讯作者：
Geim, A. K.