Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate.
Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate.
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DOI：
10.1016/s0264-410x(99)00059-6
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发表时间：
1999-06-04
期刊：
影响因子：
5.5
通讯作者：
Kang YB
中科院分区：
文献类型：
--
作者：
Kweon CH;Kwon BJ;Lee JG;Kwon GO;Kang YB
将猪流行性腹泻病毒（PEDV）野毒株在Vero细胞上连续传代。将细胞传代的PEDV命名为KPEDV-9，对其进行了新生仔猪致病性、妊娠母猪免疫原性和安全性的检测。结果表明，KPEDV-9在第93代时对新生猪的致病性降低。用减毒病毒接种的妊娠母猪通过ELISA显示出增强的免疫应答。此外，保护所递送的仔猪免受野生型PEDV的攻击。妊娠母猪的安全性试验表明，所有接种动物的产仔数均为仔猪的平均窝数。本研究结果支持了连续传代获得的弱毒可作为疫苗应用于哺乳仔猪抗PEDV感染的研究。
The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells. The cell passaged PEDV, designated KPEDV-9, was tested for its pathogenicity in the neonatal pigs, immunogenicity and safety in the pregnant sows. The result indicated that KPEDV-9 at the 93rd passage revealed reduced pathogenicity in the neonatal pigs. Pregnant sows inoculated with the attenuated virus showed increased immune responses by ELISA. In addition, delivered piglets were protected from challenge of wild type PEDV. The safety test in pregnant sows indicated that all inoculated animals farrowed the average numbers of litters of piglets. The results of this study supported that the attenuated virus derived from serial passage could be applied as vaccine for protecting suckling piglets against PEDV infection.
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影响因子：
3.7
作者：
DUARTE, M;TOBLER, K;LAUDE, H
通讯作者：
LAUDE, H
影响因子：
2.2
作者：
PIJPERS, A;VANNIEUWSTADT, AP;VERHEIJDEN, JHM
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影响因子：
1.2
作者：
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作者：
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