New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints.
New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints.
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DOI：
10.1371/journal.pone.0016512
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发表时间：
2011-01-27
期刊：
影响因子：
3.7
通讯作者：
Namba S
中科院分区：
文献类型：
--
作者：
Kawanishi T;Shiraishi T;Okano Y;Sugawara K;Hashimoto M;Maejima K;Komatsu K;Kakizawa S;Yamaji Y;Hamamoto H;Oshima K;Namba S
培养是微生物学研究中不可缺少的技术，选择性培养基培养在病原微生物的检测和从环境样品中分离商业有用的微生物中起着至关重要的作用。尽管在实验研究中已经开发了许多选择性培养基，但可能由于环境中微生物的数量巨大，非预期的微生物经常在这样的培养基上生长。在这里，我们提出了一种新的策略，用于设计基于两种选择剂，碳源和抗菌剂的高选择性培养基。我们命名为我们的策略SMART的高度选择性的中间设计算法限制两个约束。为了测试SMART方法是否适用于广泛的微生物，我们开发了用于Burkholderia glumae、Acidovorax avenae、Pectobacterium carotovorum、Ralstonia solanacearum和Xanthomonas campestris的选择性培养基。SMART开发的一系列培养基专门允许目标细菌生长。由于这些选择性培养基与已建立的选择性培养基相比对靶细菌的生长表现出高特异性，因此我们应用了三种值得注意的检测技术：基于纸的、基于流式细胞术的和基于颜色变化的检测系统用于靶细菌物种。SMART不仅有助于开发检测特定细菌的新技术，还有助于我们了解目标细菌的生态学和流行病学。
Culturing is an indispensable technique in microbiological research, and culturing with selective media has played a crucial role in the detection of pathogenic microorganisms and the isolation of commercially useful microorganisms from environmental samples. Although numerous selective media have been developed in empirical studies, unintended microorganisms often grow on such media probably due to the enormous numbers of microorganisms in the environment. Here, we present a novel strategy for designing highly selective media based on two selective agents, a carbon source and antimicrobials. We named our strategy SMART for highly Selective Medium-design Algorithm Restricted by Two constraints. To test whether the SMART method is applicable to a wide range of microorganisms, we developed selective media for Burkholderia glumae, Acidovorax avenae, Pectobacterium carotovorum, Ralstonia solanacearum, and Xanthomonas campestris. The series of media developed by SMART specifically allowed growth of the targeted bacteria. Because these selective media exhibited high specificity for growth of the target bacteria compared to established selective media, we applied three notable detection technologies: paper-based, flow cytometry-based, and color change-based detection systems for target bacteria species. SMART facilitates not only the development of novel techniques for detecting specific bacteria, but also our understanding of the ecology and epidemiology of the targeted bacteria.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
11.8
作者：
Courvalin, P;Trieu-Cuot, P
通讯作者：
Trieu-Cuot, P
影响因子：
2.8
作者：
GRANADA, GA;SEQUEIRA, L
通讯作者：
SEQUEIRA, L
影响因子：
5
作者：
Jin, M;Wang, XW;Li, JW
通讯作者：
Li, JW
影响因子：
1.2
作者：
Kawaradani, Mitsuo;Okada, Kiyotsugu;Kusakari, Shin'ichi
通讯作者：
Kusakari, Shin'ichi