Multiple histone site epigenetic modifications in nuclear transfer and in vitro fertilized bovine embryos
Multiple histone site epigenetic modifications in nuclear transfer and in vitro fertilized bovine embryos
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核移植和体外受精牛胚胎中的多个组蛋白位点表观遗传修饰
DOI：
10.1017/s0967199410000328
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发表时间：
2010-07
期刊：
影响因子：
1.7
通讯作者：
于海泉
中科院分区：
文献类型：
--
作者：
于海泉
在哺乳动物胚胎发育过程中，DNA甲基化和组蛋白修饰在基因表达调控和表观遗传重编程中起着重要作用。在克隆胚胎中，高水平的DNA甲基化和异常去甲基化在着床前被广泛观察到。体外受精（IVF）和克隆胚胎在着床前发育过程中是否存在组蛋白修饰的差异，目前尚不清楚。本研究采用间接免疫荧光和扫描共聚焦显微镜，比较了克隆和体外受精牛着床前胚胎中H3赖氨酸9、18、H4赖氨酸8、5和三甲基赖氨酸4、二甲基赖氨酸9组蛋白H3乙酰化的分布和强度规律。结果表明，克隆胚胎从原核到8细胞期，H3K9ac、H3K18ac、H4K5ac、H4K8ac、H3K4me3和H3K9me2的乙酰化和甲基化水平异常高。与体外受精对照相比，克隆胚胎中的H4K8ac和H4K5ac特别不正常。在囊胚期，克隆胚胎和体外受精胚胎之间的差异消失，所有组蛋白修饰的分布和强度模式没有明显差异。这些结果表明，在供体细胞基因组被激活之前，受体卵母细胞中的体细胞在多个位点产生了异常的组蛋白修饰。合子基因组激活后，组蛋白修饰的分布和强度模式与克隆胚胎和体外受精胚胎相当。
Summary During mammalian embryonic development, DNA methylation and histone modifications are important in gene expression regulation and epigenetic reprogramming. In cloned embryos, high levels of DNA methylation and abnormal demethylation were widely observed during the preimplantation period. Little is known whether there is a difference in histone modifications between in vitro fertilization (IVF) and cloned embryos during preimplantation development. In the present study, the distributions and intensity patterns of acetylations in H3 lysine 9, 18 and H4 lysine 8, 5 and tri-methyl lysine 4 and dimethyl-lysine 9 in histone H3 were compared in cloned and IVF bovine preimplantation embryos by using indirect immunofluorescence and scanning confocal microscopy. The results showed that the acetylation and methylation levels of H3K9ac, H3K18ac, H4K5ac, H4K8ac, H3K4me3 and H3K9me2 were abnormally high in the cloned embryos from the pronuclear to the 8-cell stage. H4K8ac and H4K5ac in the cloned embryos were particularly abnormal when compared with the IVF controls. At the blastocyst stage differences dissipated between cloned and IVF embryos and the distribution and intensity patterns of all histone modifications showed no obvious difference. These results suggest that somatic cells in recipient oocytes produced aberrant histone modifications at multiple sites before the donor cell genome is activated. After zygotic genome activation, distributions and intensity patterns of histone modifications were comparable with both cloned and IVF embryos.
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影响因子：
3.6
作者：
Wang, Fengchao;Kou, Zhaohui;Gao, Shaorong
通讯作者：
Gao, Shaorong
DOI：
10.1016/s0074-7696(08)60902-x
发表时间：
1979
期刊：
International review of cytology. Supplement
影响因子：
--
作者：
J. Gurdon;R. Laskey;E. D. De Robertis;G. Partington
通讯作者：
J. Gurdon;R. Laskey;E. D. De Robertis;G. Partington
影响因子：
3.3
作者：
Shi, L. H.;Ai, J. S.;Chen, D. Y.
通讯作者：
Chen, D. Y.
影响因子：
3.3
作者：
T. Suteevun;T. Suteevun;R. Parnpai;Sadie L. Smith;Chang Cc;S. Muenthaisong;X. Tian
通讯作者：
T. Suteevun;T. Suteevun;R. Parnpai;Sadie L. Smith;Chang Cc;S. Muenthaisong;X. Tian
影响因子：
3.6
作者：
Vigneault, C;McGraw, S;Sirard, MA
通讯作者：
Sirard, MA