Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
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Oxford Nanopore R10.4 长读长测序可从纯培养物和宏基因组中获得近乎完美的细菌基因组,无需短读长或参考修饰

DOI:
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发表时间:
2021
期刊:
bioRxiv
影响因子:
--
通讯作者:
M. Albertsen
M. Albertsen
中科院分区:
--
文献类型:
--
作者:
M. Sereika;R. Kirkegaard;S. Karst;T. Michaelsen;E. A. Sørensen;R. Wollenberg;M. Albertsen

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Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-perfect genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-perfect microbial genomes from isolates or metagenomes without shortread or reference polishing.
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