Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
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Oxford Nanopore R10.4 长读长测序可从纯培养物和宏基因组中获得近乎完美的细菌基因组,无需短读长或参考修饰
DOI:
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发表时间:
2021
期刊:
影响因子:
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通讯作者:
M. Albertsen
中科院分区:
文献类型:
--
作者:
M. Sereika;R. Kirkegaard;S. Karst;T. Michaelsen;E. A. Sørensen;R. Wollenberg;M. Albertsen
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-perfect genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-perfect microbial genomes from isolates or metagenomes without shortread or reference polishing.
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DOI:
10.1101/530824
发表时间:
2019
期刊:
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影响因子:
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作者:
De Maio N
通讯作者:
De Maio N
影响因子:
46.9
作者:
Kolmogorov, Mikhail;Yuan, Jeffrey;Pevzner, Pavel A.
通讯作者:
Pevzner, Pavel A.
影响因子:
46.9
作者:
Bishara A;Moss EL;Kolmogorov M;Parada AE;Weng Z;Sidow A;Dekas AE;Batzoglou S;Bhatt AS
通讯作者:
Bhatt AS
影响因子:
46.9
作者:
Nayfach S;Roux S;Seshadri R;Udwary D;Varghese N;Schulz F;Wu D;Paez-Espino D;Chen IM;Huntemann M;Palaniappan K;Ladau J;Mukherjee S;Reddy TBK;Nielsen T;Kirton E;Faria JP;Edirisinghe JN;Henry CS;Jungbluth SP;Chivian D;Dehal P;Wood-Charlson EM;Arkin AP;Tringe SG;Visel A;IMG/M Data Consortium;Woyke T;Mouncey NJ;Ivanova NN;Kyrpides NC;Eloe-Fadrosh EA
通讯作者:
Eloe-Fadrosh EA
DOI:
10.1007/978-1-4939-9173-0_1
发表时间:
2019-01-01
期刊:
GENE PREDICTION: METHODS AND PROTOCOLS
影响因子:
--
作者:
Chan, Patricia P.;Lowe, Todd M.
通讯作者:
Lowe, Todd M.