Molecular characterization of Peste des petits ruminants viruses in the Marmara Region of Turkey.
Molecular characterization of Peste des petits ruminants viruses in the Marmara Region of Turkey.
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DOI：
10.1111/tbed.13095
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发表时间：
2019-03
影响因子：
4.3
通讯作者：
Yilmaz H
中科院分区：
文献类型：
--
作者：
Altan E;Parida S;Mahapatra M;Turan N;Yilmaz H
最近在土耳其马尔马拉地区（包括色雷斯的欧洲部分）爆发的小反刍兽疫（PPR）非常重要，因为该地区靠近欧洲（希腊和保加利亚），PPR有可能扩散到欧洲大陆。为了研究PPRV在该地区的传播，在2011年至2012年期间，从土耳其马尔马拉地区的家养绵羊（n = 211）中采集了疑似临床和尸检样本。用真实的荧光定量RT-PCR方法在211份绵羊样品中检测到22份（10.4%）PPR病毒（PPRV）基因组，并从2只因感染死亡的绵羊肺中分离到PPR病毒。在22份阳性样本中，9份用于部分N基因扩增和测序。系统发育分析表明，该病毒属于IV系，自1999年首次官方报告以来，该病毒与土耳其东部和中部地区流行的病毒系相同。此外，还采集了100头牛的样本，以研究PPRV的潜在亚临床循环。然而，通过真实的时间RT-PCR和血清学试验发现所有动物均为阴性，表明大型反刍动物可能未暴露或感染病毒。讨论了这些发现对PPR传播到欧洲的潜在威胁的影响，包括2018年6月23日在保加利亚发生的欧洲首次PPR疫情。
Recent outbreaks of Peste des petits ruminants (PPR) in the Marmara region of Turkey including the European part of Thrace is important due to its proximity to Europe (Greece and Bulgaria) and the potential threat of spread of PPR into mainland Europe. In order to investigate the circulation of PPRV in the region suspect clinical and necropsy samples were collected from domestic sheep (n = 211) in the Marmara region of Turkey between 2011 and 2012. PPR virus (PPRV) genome was detected in 10.4% (22 out of 211) of sheep samples by real‐time RT‐PCR, and PPR virus was isolated from lungs of two sheep that died from infection. Of the 22 positive samples nine were used for partial N‐gene amplification and sequencing. The phylogenetic analyses indicated that the virus belongs to lineage IV, the same lineage that is circulating in eastern and central part of Turkey since its first official report in 1999. In addition, samples from 100 cattle were collected to investigate potential subclinical circulation of PPRV. However all were found to be negative by real‐time RT‐PCR, and also in serological tests indicating the large ruminants were likely not exposed or infected with the virus. The impact of these findings on the potential threat of spread of PPR to Europe including the first PPR outbreak in Europe in Bulgaria on 23rd June 2018 is discussed.
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影响因子：
--
作者：
Balamurugan, V.;Krishnamoorthy, P.;Rahman, H.
通讯作者：
Rahman, H.
影响因子：
3.1
作者：
Couacy-Hymann, E;Roger, F;Diallo, A
通讯作者：
Diallo, A
影响因子：
2.4
作者：
Sevik, Murat;Sait, Ahmet
通讯作者：
Sait, Ahmet
DOI：
10.1016/j.smallrumres.2016.02.018
发表时间：
2016-09
期刊：
Small ruminant research : the journal of the International Goat Association
影响因子：
--
作者：
Parida S;Muniraju M;Altan E;Baazizi R;Raj GD;Mahapatra M
通讯作者：
Mahapatra M
影响因子：
4.3
作者：
Abubakar M;Mahapatra M;Muniraju M;Arshed MJ;Khan EH;Banyard AC;Ali Q;Parida S
通讯作者：
Parida S