Molecular profiling predicts the existence of two functionally distinct classes of ovarian cancer stroma.
Molecular profiling predicts the existence of two functionally distinct classes of ovarian cancer stroma.
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DOI：
10.1155/2013/846387
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发表时间：
2013
影响因子：
--
通讯作者：
McDonald JF
中科院分区：
文献类型：
--
作者：
Lili LN;Matyunina LV;Walker LD;Benigno BB;McDonald JF
虽然间质细胞信号传导已被证明在许多癌症的进展中发挥重要作用，但对其在调节卵巢癌发展中的重要性知之甚少。本研究的目的是通过对匹配的肿瘤和周围间质组织进行分子分析，探讨卵巢癌中间质活化的过程。使用Affymetrix （U133 Plus 2.0）基因表达平台对45份组织样本进行RNA微阵列分析。采用激光捕获显微解剖（LCM）技术从卵巢癌患者的肿瘤（Cepi）和周围匹配的癌间质（CS）中分离癌细胞。作为对照，从正常（非癌）卵巢和正常间质（NS）中分离卵巢表面上皮细胞（OSE）。微阵列数据的分层聚类导致OSE， Cepi， NS和CS样本之间的明显分离。CS和Cepi样品中编码信号分子和相容受体的基因表达模式表明，存在两个亚群的癌症基质（CS），它们支持肿瘤生长的倾向不同。我们的研究结果表明，在卵巢癌患者中，微环境调节癌症发展的能力在功能上存在显著的差异。
Although stromal cell signaling has been shown to play a significant role in the progression of many cancers, relatively little is known about its importance in modulating ovarian cancer development. The purpose of this study was to investigate the process of stroma activation in human ovarian cancer by molecular analysis of matched sets of cancer and surrounding stroma tissues. RNA microarray profiling of 45 tissue samples was carried out using the Affymetrix (U133 Plus 2.0) gene expression platform. Laser capture microdissection (LCM) was employed to isolate cancer cells from the tumors of ovarian cancer patients (Cepi) and matched sets of surrounding cancer stroma (CS). For controls, ovarian surface epithelial cells (OSE) were isolated from the normal (noncancerous) ovaries and normal stroma (NS). Hierarchical clustering of the microarray data resulted in clear separations between the OSE, Cepi, NS, and CS samples. Expression patterns of genes encoding signaling molecules and compatible receptors in the CS and Cepi samples indicate the existence of two subgroups of cancer stroma (CS) with different propensities to support tumor growth. Our results indicate that functionally significant variability exists among ovarian cancer patients in the ability of the microenvironment to modulate cancer development.
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