Label-free in vivo cellular-level detection and imaging of apoptosis.
Label-free in vivo cellular-level detection and imaging of apoptosis.
复制标题
无标记的体内细胞级检测和凋亡的成像。
DOI：
10.1002/jbio.201600003
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发表时间：
2017-01
影响因子：
2.8
通讯作者：
Boppart, Stephen A.
中科院分区：
文献类型：
--
作者：
Bower, Andrew J.;Marjanovic, Marina;Zhao, Youbo;Li, Joanne;Chaney, Eric J.;Boppart, Stephen A.
细胞死亡在健康和体内平衡以及在广泛疾病的发病机制和治疗中起关键作用，并且可以大致分为两个主要类别：细胞凋亡或程序性细胞死亡，以及坏死或急性细胞死亡。虽然这些过程已在体外广泛表征，但在体内细胞水平上的细胞凋亡和坏死的无标记检测尚未显示。在这项研究中，第一次，荧光寿命成像显微镜（FLIM）的细胞内还原型烟酰胺腺嘌呤二核苷酸（NADH）被用来评估在体内小鼠表皮角质形成细胞诱导凋亡和坏死后的代谢反应。结果显示，与坏死组织相比，凋亡组织中NADH的平均寿命和蛋白质结合与游离NADH的细胞内比率均显著升高。此外，这两种细胞死亡过程的纵向轮廓显示出显着差异。通过识别和提取这些时间代谢特征，可以在活生物体内的天然组织环境中研究单细胞中的凋亡。细胞凋亡或程序性细胞死亡在健康和稳态以及许多疾病的治疗中起着关键作用。虽然这一过程已在体外广泛表征，但体内细胞的无标记检测尚未显示。在这项研究中，减少烟酰胺腺嘌呤二核苷酸的荧光寿命成像显微镜被用来评估体内小鼠角质形成细胞诱导凋亡后的代谢反应。
Cell death plays a critical role in health and homeostasis as well as in the pathogenesis and treatment of a broad spectrum of diseases and can be broadly divided into two main categories: apoptosis, or programmed cell death, and necrosis, or acute cell death. While these processes have been characterized extensively in vitro, label-free detection of apoptosis and necrosis at the cellular level in vivo has yet to be shown. In this study, for the first time, fluorescence lifetime imaging microscopy (FLIM) of intracellular reduced nicotinamide adenine dinucleotide (NADH) was utilized to assess the metabolic response of in vivo mouse epidermal keratinocytes following induction of apoptosis and necrosis. Results show significantly elevated levels of both the mean lifetime of NADH and the intracellular ratio of protein bound-to-free NADH in the apoptotic compared to the necrotic tissue. In addition, the longitudinal profiles of these two cell death processes show remarkable differences. By identifying and extracting these temporal metabolic signatures, apoptosis in single cells can be studied in native tissue environments within the living organism. Apoptosis, or programmed cell death plays a critical role in health and homeostasis as well as in the treatment of many diseases. While this process has been characterized extensively in vitro, label-free detection of cells in vivo has yet to be shown. In this study, fluorescence lifetime imaging microscopy of reduced nicotinamide adenine dinucleotide is utilized to assess the metabolic response of in vivo mouse keratinocytes following induction of apoptosis.
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影响因子：
2.7
作者：
Buschke DG;Squirrell JM;Fong JJ;Eliceiri KW;Ogle BM
通讯作者：
Ogle BM
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
82.9
作者：
Narula, J;Acio, ER;Strauss, HW
通讯作者：
Strauss, HW
影响因子：
3.5
作者：
Levitt, Jonathan M.;Baldwin, Amy;Georgakoudi, Irene
通讯作者：
Georgakoudi, Irene
影响因子：
3.5
作者：
Farhat, Golnaz;Mariampillai, Adrian;Kolios, Michael C.
通讯作者：
Kolios, Michael C.