Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin are targets of miR-644a.
Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin are targets of miR-644a.
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DOI：
10.1371/journal.pone.0047510
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Shukla GC
中科院分区：
文献类型：
--
作者：
Sikand K;Singh J;Ebron JS;Shukla GC
microRNA（miRNA）对其靶mRNA表达的过表达或下调的结果通常通过使用适当的管家基因作为内部对照的逆转录和定量PCR分析来验证。miRNA对管家基因表达可能的直接或间接影响往往被忽视。在许多管家基因中，甘油醛-3-磷酸脱氢酶（GAPDH）和β-肌动蛋白的表达已被广泛用于基因表达数据的标准化。在这里，我们表明GAPDH和β-actin是miR-644 a的直接靶点。我们的数据表明GAPDH和β-actin不适合作为miR-644 a功能研究的内部对照，并强调需要仔细考虑miRNA实验中参考基因的选择。
Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expression of housekeeping genes are often overlooked. Among many housekeeping genes, expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin have been used extensively for normalization of gene expression data. Here, we show that GAPDH and β-actin are direct targets of miR-644a. Our data demonstrate the unsuitability of GAPDH and β-actin as internal controls in miR-644a functional studies and emphasize the need to carefully consider the choice of a reference gene in miRNA experiments.
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影响因子：
2.9
作者：
GLASER, PE;GROSS, RW
通讯作者：
GROSS, RW
DOI：
10.1073/pnas.93.18.9937
发表时间：
1996-09-03
影响因子：
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