Acquired hydrocephalus is associated with neuroinflammation, progenitor loss, and cellular changes in the subventricular zone and periventricular white matter.
Acquired hydrocephalus is associated with neuroinflammation, progenitor loss, and cellular changes in the subventricular zone and periventricular white matter.
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DOI：
10.1186/s12987-022-00313-3
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发表时间：
2022-02-22
影响因子：
7.3
通讯作者：
McAllister JP 2nd
中科院分区：
文献类型：
--
作者：
Garcia-Bonilla M;Castaneyra-Ruiz L;Zwick S;Talcott M;Otun A;Isaacs AM;Morales DM;Limbrick DD Jr;McAllister JP 2nd
脑积水是一种神经系统疾病，在美国每 100,000 名新生儿中就有 80-125 人患有脑积水。神经病理学包括脑室扩大、脑室周围白质 (PVWM) 改变、炎症和神经胶质增生。我们假设猪模型中的脑积水与室下和 PVWM 细胞改变以及神经炎症有关，这可能类似于脑积水婴儿中描述的神经病理学。通过向 35 日龄母猪脑池内注射高岭土诱导脑积水（组织分析 n = 7，脑脊液分析 n = 10）。年龄匹配的假对照组接受盐水注射（n = 6）。 19-40 天后，进行 MRI 扫描以测量心室容积。在室下区 (SVZ) 中研究干细胞增殖，并在 PVWM 中检查细胞死亡和少突胶质细胞。通过定量 PVWM 中的星形胶质细胞和小胶质细胞以及 CSF 中的炎症细胞因子来研究神经炎症反应。脑室扩张在侧脑室体中尤其明显，其中发生室管膜破裂。 PVWM 显示细胞死亡增加 44%，少突胶质细胞减少 67%。在SVZ中，增殖细胞和少突胶质细胞的数量分别减少了75%和57%。 SVZ 面积的减小与心室容量的增加显着相关。脑积水猪发生神经炎症，PVWM 中星形胶质细胞和小胶质细胞显着增加，脑脊液中炎症白细胞介素 IL-6 和 IL-8 水平升高。获得性脑积水的诱导会导致 PVWM 的改变、SVZ 细胞增殖的减少以及神经炎症。在线版本包含可在 10.1186/s12987-022-00313-3 获取的补充材料。
Hydrocephalus is a neurological disease with an incidence of 80–125 per 100,000 births in the United States. Neuropathology comprises ventriculomegaly, periventricular white matter (PVWM) alterations, inflammation, and gliosis. We hypothesized that hydrocephalus in a pig model is associated with subventricular and PVWM cellular alterations and neuroinflammation that could mimic the neuropathology described in hydrocephalic infants. Hydrocephalus was induced by intracisternal kaolin injections in 35-day old female pigs (n = 7 for tissue analysis, n = 10 for CSF analysis). Age-matched sham controls received saline injections (n = 6). After 19–40 days, MRI scanning was performed to measure the ventricular volume. Stem cell proliferation was studied in the Subventricular Zone (SVZ), and cell death and oligodendrocytes were examined in the PVWM. The neuroinflammatory reaction was studied by quantifying astrocytes and microglial cells in the PVWM, and inflammatory cytokines in the CSF. The expansion of the ventricles was especially pronounced in the body of the lateral ventricle, where ependymal disruption occurred. PVWM showed a 44% increase in cell death and a 67% reduction of oligodendrocytes. In the SVZ, the number of proliferative cells and oligodendrocyte decreased by 75% and 57% respectively. The decrease of the SVZ area correlated significantly with ventricular volume increase. Neuroinflammation occurred in the hydrocephalic pigs with a significant increase of astrocytes and microglia in the PVWM, and high levels of inflammatory interleukins IL-6 and IL-8 in the CSF. The induction of acquired hydrocephalus produced alterations in the PVWM, reduced cell proliferation in the SVZ, and neuroinflammation. The online version contains supplementary material available at 10.1186/s12987-022-00313-3.
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影响因子：
2.9
作者：
Costine BA;Missios S;Taylor SR;McGuone D;Smith CM;Dodge CP;Harris BT;Duhaime AC
通讯作者：
Duhaime AC
影响因子：
3.3
作者：
Gonzalez-Perez, O.;Gutierrez-Fernandez, F.;Lopez-Virgen, V.;Collas-Aguilar, J.;Quinones-Hinojosa, A.;Garcia-Verdugo, J. M.
通讯作者：
Garcia-Verdugo, J. M.
DOI：
10.1098/rspb.1967.0002
发表时间：
1967-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DICKERSON, JW;DOBBING, J
通讯作者：
DOBBING, J
影响因子：
5.3
作者：
Campos-Ordonez, Tania;Herranz-Perez, Vicente;Gonzalez-Perez, Oscar
通讯作者：
Gonzalez-Perez, Oscar
DOI：
10.1097/01.jnen.0000171648.86718.bb
发表时间：
2005-07-01
影响因子：
3.2
作者：
Domínguez-Pinos, MD;Páez, P;Rodríguez, EM
通讯作者：
Rodríguez, EM