In Vitro Regeneration, Ex Vitro Rooting and Foliar Stoma Studies of Pseudostellaria heterophylla (Miq.) Pax
In Vitro Regeneration, Ex Vitro Rooting and Foliar Stoma Studies of Pseudostellaria heterophylla (Miq.) Pax
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太子参的体外再生、离体生根和叶造口研究
DOI：
10.3390/agronomy10070949
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发表时间：
2020-07
期刊：
影响因子：
--
通讯作者：
Boling Liu
中科院分区：
文献类型：
--
作者：
Fengyun Wang;Xiaowei Xin;Hao Wei;Xiaohui Qiu;Boling Liu
太子参（Pseudostellaria heterophylla）为菊科太子参属植物，是一种重要的中草药，常用于治疗儿童多种疾病，具有观赏价值。在这项研究中，从野生植物的节段，并作为外植体，以开发一个有效的微繁殖协议，该物种。MS +1.5 mg·L−1 6-苄基腺嘌呤（6-BA）培养基对腋芽的诱导和促进生长效果最好，MS +0.1 mg·L−1吲哚-3-丁酸（IBA）培养基对腋芽的诱导生根效果最好。为了减少劳动力，时间和成本，试管苗生根条件下。用100 mg·L−1萘乙酸（NAA）预处理1 min，生根率为86.7%。比较离体生根苗和离体生根苗叶片的显微结构，发现前者气孔器异常。离体苗气孔器正常，但气孔密度降低，说明离体苗更能适应田间环境条件。我们确定了最佳的培养基，以提高试管苗的生根效率。本研究结果对异叶松的农业栽培具有一定的指导意义。
Pseudostellaria heterophylla, in the family Caryophyllaceae, is an important Chinese medicinal plant commonly used to treat various diseases in children and valued for its ornamental properties. In this study, nodal segments were obtained from wild plants and used as explants to develop an efficient micropropagation protocol for this species. Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 6-benzyladenine (6-BA) was the most suitable medium for inducing axillary buds and enhancing their growth, and MS medium containing 0.1 mg·L−1 indole-3-butyric acid (IBA) was the most effective for inducing in vitro rooting. To reduce labor, time, and cost, microshoots were rooted under ex vitro conditions. Pretreatments of the shoots with 100 mg·L−1 naphthaleneacetic acid (NAA) for 1 min ensured successful rooting in 86.7% of shoots. Comparison of the leaf microstructure between in vitro- and ex vitro-rooted plantlets revealed abnormal stomatal apparatus in the former. The stomatal apparatus of ex vitro plantlets were normal, although the stomatal density was reduced, which indicated that these plantlets were more likely to be able to adapt to environmental conditions in the field. We identified the optimal medium for P. heterophylla multiplication with respect to increased rooting efficiency of micropropagated shoots under ex vitro conditions. This results presented here will be helpful for agricultural cultivation of P. heterophylla.
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影响因子：
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作者：
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DOI：
10.1007/s11240-009-9534-x
发表时间：
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期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
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DOI：
--
发表时间：
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影响因子：
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