Resolvin D2 Relieving Radicular Pain is Associated with Regulation of Inflammatory Mediators, Akt/GSK-3β Signal Pathway and GPR18
Resolvin D2 Relieving Radicular Pain is Associated with Regulation of Inflammatory Mediators, Akt/GSK-3β Signal Pathway and GPR18
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Resolvin D2 缓解根性疼痛与炎症介质、Akt/GSK-3β 信号通路和 GPR18 的调节有关
DOI：
10.1007/s11064-018-2666-9
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发表时间：
2018-11
影响因子：
4.4
通讯作者：
Tao Sun
中科院分区：
文献类型：
--
作者：
Lan-Yu Zhang;Zhi-Hua Liu;Qing Zhu;Shuang Wen;Cong-Xian Yang;Zhi-Jian Fu;Tao Sun
Neuroinflammation induced by protruded nucleus pulposus (NP) has been shown to play a significant role in facilitation of radicular pain. Resolvin D2 (RvD2), a novel member of resolvin family, exhibits potent anti-inflammatory, pro-resolving and antinociceptive effects. But the effect of RvD2 in radicular pain remains unknown. The radicular pain rat models were induced by application of NP to L5 dorsal root ganglion. Each animal received intrathecal injections of vehicle or RvD2 (10 ng µl−1or 100 ng µl−1). Mechanical thresholds were determined by measuring the paw withdrawal threshold for 7 days. The expressions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and transforming growth factor-β1 (TGF-β1) in ipsilateral lumbar segment of rat spinal dorsal horns were measured by using ELISA and real time-PCR. Western blot was used to measure the expressions of phosphorylated Akt (p-Akt) and phosphorylated glycogen synthase kinase 3 beta (p-GSK-3β). The expressions and distributions of RvD2 receptor, G-protein-coupled receptor 18 (GPR18), were also explored in the spinal cord of rats by using double-label immunofluorescence. RvD2 treatment caused significant reductions in the intensity of mechanical hypersensitivity and spinal expressions of TNF-α and IL-6. Meanwhile, RvD2 increased the expressions of TGF-β1 and regulated Akt/GSK-3β signaling. Furthermore, immunofluorescence showed that GPR18 colocalized with neurons and astrocytes in spinal cord. The results suggested that RvD2 might attenuate mechanical allodynia via regulating the expressions of inflammatory mediators and activation of Akt/GSK-3β signal pathway. RvD2 might offer a hopeful method for radicular pain therapy.
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影响因子：
15.9
作者：
Ji RR;Xu ZZ;Strichartz G;Serhan CN
通讯作者：
Serhan CN
Interleukin-6：一种新兴的病理性疼痛调节剂
影响因子：
9.3
作者：
Zhou YQ;Liu Z;Liu ZH;Chen SP;Li M;Shahveranov A;Ye DW;Tian YK
通讯作者：
Tian YK
DOI：
10.1084/jem.20150225
发表时间：
2015-07-27
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Chiang N;Dalli J;Colas RA;Serhan CN
通讯作者：
Serhan CN
影响因子：
3.3
作者：
Echeverry S;Shi XQ;Haw A;Liu H;Zhang ZW;Zhang J
通讯作者：
Zhang J
影响因子：
15.9
作者：
Wang, Huizhi;Kumar, Akhilesh;Lamont, Richard J.;Scott, David A.
通讯作者：
Scott, David A.