Full genome sequencing and genetic characterization of Eubenangee viruses identify Pata virus as a distinct species within the genus Orbivirus.
Full genome sequencing and genetic characterization of Eubenangee viruses identify Pata virus as a distinct species within the genus Orbivirus.
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DOI：
10.1371/journal.pone.0031911
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Mertens PP
中科院分区：
文献类型：
--
作者：
Belaganahalli MN;Maan S;Maan NS;Nomikou K;Pritchard I;Lunt R;Kirkland PD;Attoui H;Brownlie J;Mertens PP
Eubenangee病毒先前已被确定为Tammar猝死综合征（TSDS）的原因。Eubenangee病毒（EUBV）、Tilligery病毒（TILV）、Pata病毒（PATAV）和Ngoupe病毒（NGOV）目前都被分类在呼肠孤病毒科环状病毒属的Eubenangee病毒种内。全基因组测序证实，EUBV和TILV（均来自澳大利亚）在保守的聚合酶VP 1（Pol）、亚核心VP 3（T2）和外核心VP 7（T13）蛋白中显示出高水平的氨基酸序列同一性（>92%），因此被适当地分类为相同的病毒物种。然而，它们在其较大的外衣壳蛋白VP 2中显示出低得多的氨基酸（aa）同一性水平（<53%），与两种不同血清型- EUBV-1和EUBV-2（分别）的成员一致。相比之下，PATAV显示与EUBV或TILV的显著较低水平的aa序列同一性（在VP 1（Pol）和VP 3（T2）中具有<71%，并且在VP 7（T13）中具有<57%的aa同一性），与不同病毒物种的成员一致。因此，已向ICTV的呼肠孤病毒科研究组提交了一份建议，将“帕塔病毒”识别为一种新的环状病毒属，其中PATAV分离株为血清型1（PATAV-1）。在其他环状病毒中，PATAV显示与流行性出血症病毒（EHDV）最接近的关系，其VP 3（T2）、VP 1（Pol）和VP 7（T13）的氨基酸同源性分别为80.7%、72.4%和66.9%。虽然Ngoupe病毒无法用于这些研究，但与PATAV一样，它也是在中非分离的，因此似乎也属于新物种，可能是一个独特的“类型”。所提供的数据将有助于诊断试验设计和这些病毒的其他分离株的鉴定。
Eubenangee virus has previously been identified as the cause of Tammar sudden death syndrome (TSDS). Eubenangee virus (EUBV), Tilligery virus (TILV), Pata virus (PATAV) and Ngoupe virus (NGOV) are currently all classified within the Eubenangee virus species of the genus Orbivirus, family Reoviridae. Full genome sequencing confirmed that EUBV and TILV (both of which are from Australia) show high levels of aa sequence identity (>92%) in the conserved polymerase VP1(Pol), sub-core VP3(T2) and outer core VP7(T13) proteins, and are therefore appropriately classified within the same virus species. However, they show much lower amino acid (aa) identity levels in their larger outer-capsid protein VP2 (<53%), consistent with membership of two different serotypes - EUBV-1 and EUBV-2 (respectively). In contrast PATAV showed significantly lower levels of aa sequence identity with either EUBV or TILV (with <71% in VP1(Pol) and VP3(T2), and <57% aa identity in VP7(T13)) consistent with membership of a distinct virus species. A proposal has therefore been sent to the Reoviridae Study Group of ICTV to recognise ‘Pata virus’ as a new Orbivirus species, with the PATAV isolate as serotype 1 (PATAV-1). Amongst the other orbiviruses, PATAV shows closest relationships to Epizootic Haemorrhagic Disease virus (EHDV), with 80.7%, 72.4% and 66.9% aa identity in VP3(T2), VP1(Pol), and VP7(T13) respectively. Although Ngoupe virus was not available for these studies, like PATAV it was isolated in Central Africa, and therefore seems likely to also belong to the new species, possibly as a distinct ‘type’. The data presented will facilitate diagnostic assay design and the identification of additional isolates of these viruses.
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影响因子：
3.8
作者：
BORDEN, EC;SHOPE, RE;MURPHY, FA
通讯作者：
MURPHY, FA
DOI：
10.4269/ajtmh.1973.22.551
发表时间：
1973-01-01
影响因子：
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作者：
GARD, G;MARSHALL, ID;WOODROOFE, GM
通讯作者：
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影响因子：
3.8
作者：
GONZALEZ, HA;KNUDSON, DL
通讯作者：
KNUDSON, DL
影响因子：
5
作者：
Anthony, S. J.;Maan, S.;Mertens, P. P. C.
通讯作者：
Mertens, P. P. C.
DOI：
10.1016/b978-012369368-6.50007-1
发表时间：
2009-01-01
期刊：
BLUETONGUE
影响因子：
--
作者：
Attoui, Houssam;Maan, Sushila;Mertens, Peter P. C.
通讯作者：
Mertens, Peter P. C.