PpSP32-like protein as a marker of human exposure to Phlebotomus argentipes in Leishmania donovani foci in Bangladesh.
PpSP32-like protein as a marker of human exposure to Phlebotomus argentipes in Leishmania donovani foci in Bangladesh.
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DOI：
10.1016/j.ijpara.2021.05.006
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发表时间：
2021-11
影响因子：
4
通讯作者：
Volf P
中科院分区：
文献类型：
--
作者：
Sumova P;Sanjoba C;Willen L;Polanska N;Matsumoto Y;Noiri E;Paul SK;Ozbel Y;Volf P
银足白蛉是杜氏利什曼原虫在印度次大陆的唯一传播媒介。研究区域40%的人具有抗银足鼠唾液的IgG抗体。发现针对银足拟杆菌唾液的IgG应答与rPagSP06之间存在相关性。rPagSP06是测量人类暴露于银足并殖吸虫的有效抗原。银足白蛉是杜氏利什曼原虫的主要媒介，杜氏利什曼原虫是在印度次大陆引起内脏利什曼病的原生动物寄生虫。白蛉唾液可诱导被银足白蛉叮咬的宿主产生特异性抗唾液蛋白抗体。在这里，我们利用这些抗体作为人类暴露于P. argentipes在内脏利什曼病流行区在帕布纳区，孟加拉国的标记。使用整个唾液腺匀浆作为抗原来检测这些抗体有几个局限性，因此它正在被特定重组唾液蛋白的使用所取代。我们已经鉴定了被咬伤人血清识别的三种主要的银足并殖吸虫唾液抗原蛋白，以重组形式（rPagSP04、rPagSP05和rPagSP06）表达它们，并测试它们在ELISA和免疫印迹中的适用性。其中之一，PpSP32样蛋白rPagSP06，被确定为最有希望的抗原，显示出最高的相似性和相关性的IgG反应，以P. argentipes唾液腺匀浆。此外，我们已经验证了rPagSP06在585个个体的大队列中的适用性，并获得了抗rPagSP06和抗银足贝唾液IgG应答的高相关系数。抗rPagSP06和抗银足贝氏菌唾液腺匀浆IgG应答遵循相似的右偏分布。本文首次报道了用重组唾液蛋白筛选人血清中抗银足并殖吸虫唾液抗体。rPagSP06被证明是一种有效的抗原，用于在内脏利什曼病流行区筛选暴露于银足疟原虫叮咬的人血清。
Phlebotomus argentipes is a sole vector of Leishmania donovani in the Indian subcontinent. 40% of humans in the study area have IgG antibodies against P. argentipes saliva. A correlation was found between IgG responses against P. argentipes saliva and rPagSP06. rPagSP06 is a valid antigen to measure human exposure to P. argentipes. Phlebotomus argentipes is a predominant vector of Leishmania donovani, the protozoan parasite causing visceral leishmaniasis in the Indian subcontinent. In hosts bitten by P. argentipes, sand fly saliva elicits the production of specific anti-salivary protein antibodies. Here, we have utilised these antibodies as markers of human exposure to P. argentipes in a visceral leishmaniasis endemic area in Pabna district, Bangladesh. The use of whole salivary gland homogenate as an antigen to detect these antibodies has several limitations, therefore it is being superseded by the use of specific recombinant salivary proteins. We have identified three major P. argentipes salivary antigenic proteins recognised by sera of bitten humans, expressed them in a recombinant form (rPagSP04, rPagSP05 and rPagSP06) and tested their applicability in ELISA and immunoblot. One of them, PpSP32-like protein rPagSP06, was identified as the most promising antigen, showing highest resemblance and correlation with the IgG response to P. argentipes salivary gland homogenate. Furthermore, we have validated the applicability of rPagSP06 in a large cohort of 585 individuals and obtained a high correlation coefficient for anti-rPagSP06 and anti-P. argentipes saliva IgG responses. The anti-rPagSP06 and anti-P. argentipes salivary gland homogenate IgG responses followed a similar right-skewed distribution. This is the first report of screening human sera for anti-P. argentipes saliva antibodies using recombinant salivary protein. The rPagSP06 was proven to be a valid antigen for screening human sera for exposure to P. argentipes bites in a visceral leishmaniasis endemic area.
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影响因子：
4
作者：
Ghosh, KN;Mukhopadhyay, J
通讯作者：
Mukhopadhyay, J
影响因子：
3.8
作者：
Chowdhury, Rajib;Chowdhury, Vashkar;Banu, Qamar
通讯作者：
Banu, Qamar
影响因子：
1.9
作者：
Kostalova, T.;Lestinova, T.;Volf, P.
通讯作者：
Volf, P.
影响因子：
4.3
作者：
Julenius, K;Molgaard, A;Brunak, S
通讯作者：
Brunak, S
DOI：
10.4269/ajtmh.2010.09-0336
发表时间：
2010-05-01
影响因子：
3.3
作者：
Clements, Meredith F.;Gidwani, Kamlesh;Rogers, Matthew E.
通讯作者：
Rogers, Matthew E.