Mining of Brassica-Specific Genes (BSGs) and Their Induction in Different Developmental Stages and under Plasmodiophora brassicae Stress in Brassica rapa.
Mining of Brassica-Specific Genes (BSGs) and Their Induction in Different Developmental Stages and under Plasmodiophora brassicae Stress in Brassica rapa.
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甘蓝型甘蓝特异性基因（BSG）的挖掘及其在不同发育阶段和甘蓝根霉胁迫下的诱导
DOI：
10.3390/ijms19072064
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发表时间：
2018-07-16
影响因子：
5.6
通讯作者：
Piao Z
中科院分区：
文献类型：
--
作者：
Jiang M;Dong X;Lang H;Pang W;Zhan Z;Li X;Piao Z
孤儿基因又称谱系特异性基因（lineage-specific gene，LSGs），在植物对生物和非生物胁迫的应答中起着重要作用，并与谱系特异性结构和生物学功能相关。到目前为止，还没有研究调查基因数量，基因特征，或基因表达模式的孤儿基因在白菜。本研究以芜菁基因组为基础，鉴定了1540个油菜属特异基因（BSGs）和1824个十字花科特异基因（CSGs）。基因特征分析表明，BSG和CSG的多外显子基因比例较低，GC含量较高，基因长度较短。此外，还获得了5种BSG，并对51种物种的529个真实的A亚基因组特异性BSG中的145个进行了PCR验证。通过计算机模拟和半定量PCR，BSG的基因表达分析表明BSG在各种组织中表达，并且可以由芸苔根肿菌诱导。此外，一个A/C亚基因组特异的BSG，BSGs 1，在抽穗期特异表达，表明该基因可能与叶球的形成。这些结果为进一步研究BSGs在B中对油菜特异表型和生物胁迫的分子功能提供了有价值的生物学信息。拉帕。
Orphan genes, also called lineage-specific genes (LSGs), are important for responses to biotic and abiotic stresses, and are associated with lineage-specific structures and biological functions. To date, there have been no studies investigating gene number, gene features, or gene expression patterns of orphan genes in Brassica rapa. In this study, 1540 Brassica-specific genes (BSGs) and 1824 Cruciferae-specific genes (CSGs) were identified based on the genome of Brassica rapa. The genic features analysis indicated that BSGs and CSGs possessed a lower percentage of multi-exon genes, higher GC content, and shorter gene length than evolutionary-conserved genes (ECGs). In addition, five types of BSGs were obtained and 145 out of 529 real A subgenome-specific BSGs were verified by PCR in 51 species. In silico and semi-qPCR, gene expression analysis of BSGs suggested that BSGs are expressed in various tissue and can be induced by Plasmodiophora brassicae. Moreover, an A/C subgenome-specific BSG, BSGs1, was specifically expressed during the heading stage, indicating that the gene might be associated with leafy head formation. Our results provide valuable biological information for studying the molecular function of BSGs for Brassica-specific phenotypes and biotic stress in B. rapa.
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影响因子：
4.4
作者：
Johnson BR;Tsutsui ND
通讯作者：
Tsutsui ND
影响因子：
5.2
作者：
Chen, Jingjing;Piao, Yinglan;Piao, Zhongyun
通讯作者：
Piao, Zhongyun
影响因子：
7.2
作者：
Guo, Ya-Long
通讯作者：
Guo, Ya-Long
影响因子：
--
作者：
Guo WJ;Li P;Ling J;Ye SP
通讯作者：
Ye SP
影响因子：
11.6
作者：
Kagale, Sateesh;Robinson, Stephen J.;Parkin, Isobel A. P.
通讯作者：
Parkin, Isobel A. P.