Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening.
Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening.
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DOI：
10.1007/s11626-010-9303-5
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发表时间：
2010-04
影响因子：
2.1
通讯作者：
Lavon, Neta
中科院分区：
文献类型：
--
作者：
Narwani, Kavita;Biancotti, Juan-Carlos;Golan-Lev, Tamar;Buehler, Nicole;Hill, David;Shifman, Sagiv;Benvenisty, Nissim;Lavon, Neta
关键词：
人类胚胎干细胞来源于植入前胚胎的内细胞团。细胞具有无限的增殖潜力和分化成三个胚层细胞的能力。人类胚胎干细胞用于研究人类胚胎发生和疾病建模，并且在未来可能用作细胞治疗和药物筛选的细胞。人类胚胎干细胞通常从剩余的正常冷冻胚胎中分离，建议从植入前遗传学诊断发现的患病胚胎中分离。在这里，我们报告了12个人胚胎干细胞系的分离和他们的彻底表征。这些品系来源于通过植入前遗传筛选检测到具有非整倍体的胚胎。这些细胞系的核型分析表明，它们是整倍体，具有46条染色体。我们的解释是，整倍体细胞起源于嵌合体胚胎，并在体外选择有利于整倍体细胞。未分化的细胞表现出长期增殖，并表达胚胎干细胞的典型标志物，如OCT 4、NANOG和TRA-1-60。细胞在体内和体外均表现出多能分化。为了进一步描述不同的线，我们分析了他们的种族起源和家庭之间的关系。上述结果使我们得出结论，非整倍体嵌合体胚胎，注定要被丢弃，可以作为正常的整倍体人胚胎干细胞系的来源。这些线代表不同的种族群体;需要更多的线来代表所有人口。
Human embryonic stem cells are derived from the inner cell mass of pre-implantation embryos. The cells have unlimited proliferation potential and capacity to differentiate into the cells of the three germ layers. Human embryonic stem cells are used to study human embryogenesis and disease modeling and may in the future serve as cells for cell therapy and drug screening. Human embryonic stem cells are usually isolated from surplus normal frozen embryos and were suggested to be isolated from diseased embryos detected by pre-implantation genetic diagnosis. Here we report the isolation of 12 human embryonic stem cell lines and their thorough characterization. The lines were derived from embryos detected to have aneuploidy by pre-implantation genetic screening. Karyotype analysis of these cell lines showed that they are euploid, having 46 chromosomes. Our interpretation is that the euploid cells originated from mosaic embryos, and in vitro selection favored the euploid cells. The undifferentiated cells exhibited long-term proliferation and expressed markers typical for embryonic stem cells such as OCT4, NANOG, and TRA-1-60. The cells manifested pluripotent differentiation both in vivo and in vitro. To further characterize the different lines, we have analyzed their ethnic origin and the family relatedness among them. The above results led us to conclude that the aneuploid mosaic embryos that are destined to be discarded can serve as source for normal euploid human embryonic stem cell lines. These lines represent various ethnic groups; more lines are needed to represent all populations.
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影响因子：
23.9
作者：
Eiges, Rachel;Urbach, Achia;Ben-Yosef, Dalit
通讯作者：
Ben-Yosef, Dalit
影响因子：
56.9
作者：
Thomson, JA;Itskovitz-Eldor, J;Jones, JM
通讯作者：
Jones, JM
影响因子：
4
作者：
Verlinsky, Y;Strelchenko, N;Kuliev, A
通讯作者：
Kuliev, A
影响因子：
46.9
作者：
Adewumi, Oluseun;Aflatoonian, Behrouz;Zhang, Weidong
通讯作者：
Zhang, Weidong
影响因子：
5.7
作者：
Itskovitz-Eldor, J;Schuldiner, M;Benvenisty, N
通讯作者：
Benvenisty, N