Global transcriptomic analysis suggests carbon dioxide as an environmental stressor in spaceflight: A systems biology GeneLab case study.
Global transcriptomic analysis suggests carbon dioxide as an environmental stressor in spaceflight: A systems biology GeneLab case study.
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DOI：
10.1038/s41598-018-22613-1
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发表时间：
2018-03-08
影响因子：
4.6
通讯作者：
Costes SV
中科院分区：
文献类型：
--
作者：
Beheshti A;Cekanaviciute E;Smith DJ;Costes SV
太空飞行引入了一系列环境压力因素，包括微重力、电离辐射、饮食变化和大气气体成分的改变。为了了解每个环境因素对宇航员的影响，必须单独调查潜在的影响。啮齿类动物太空飞行实验包括标准的动物笼和动物围护模块（AEMs）， AEMs是用于在太空飞行中容纳啮齿动物的笼子。地面控制aem的设计是为了适应航天环境。有有限的研究检查的生物反应总是由于配置的AEM和动物住房。为了研究与太空飞行匹配的AEM中啮齿类动物的先天全局转录组模式，与标准的动物笼相比，我们利用了美国宇航局基因实验室存储库的公开数据。使用系统生物学方法，我们观察到AEM住房与显著的转录组差异相关，包括代谢减少、免疫反应改变和可能的致瘤途径的激活。虽然我们没有进行任何功能研究，但我们的发现揭示了AEM的轻度缺氧表型，可能是由于大气中的二氧化碳增加以匹配太空飞行的条件。我们的调查说明了产生新假设的过程，并通过重新利用多个太空飞行数据集为未来的实验研究提供信息。
Spaceflight introduces a combination of environmental stressors, including microgravity, ionizing radiation, changes in diet and altered atmospheric gas composition. In order to understand the impact of each environmental component on astronauts it is important to investigate potential influences in isolation. Rodent spaceflight experiments involve both standard vivarium cages and animal enclosure modules (AEMs), which are cages used to house rodents in spaceflight. Ground control AEMs are engineered to match the spaceflight environment. There are limited studies examining the biological response invariably due to the configuration of AEM and vivarium housing. To investigate the innate global transcriptomic patterns of rodents housed in spaceflight-matched AEM compared to standard vivarium cages we utilized publicly available data from the NASA GeneLab repository. Using a systems biology approach, we observed that AEM housing was associated with significant transcriptomic differences, including reduced metabolism, altered immune responses, and activation of possible tumorigenic pathways. Although we did not perform any functional studies, our findings revealed a mild hypoxic phenotype in AEM, possibly due to atmospheric carbon dioxide that was increased to match conditions in spaceflight. Our investigation illustrates the process of generating new hypotheses and informing future experimental research by repurposing multiple space-flown datasets.
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影响因子：
11.2
作者：
Beheshti A;Benzekry S;McDonald JT;Ma L;Peluso M;Hahnfeldt P;Hlatky L
通讯作者：
Hlatky L
影响因子：
2.6
作者：
Chen, Heng;Shen, Yunfeng;Zhang, Ri
通讯作者：
Zhang, Ri
DOI：
10.1097/jom.0000000000000158
发表时间：
2014-05-01
影响因子：
3.2
作者：
Law, Jennifer;Van Baalen, Mary;Alexander, David
通讯作者：
Alexander, David
影响因子：
7.3
作者：
Fernandez-Gonzalo R;Baatout S;Moreels M
通讯作者：
Moreels M
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J