Streptococcus mutans induces IgA nephropathy-like glomerulonephritis in rats with severe dental caries.
Streptococcus mutans induces IgA nephropathy-like glomerulonephritis in rats with severe dental caries.
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DOI：
10.1038/s41598-021-85196-4
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发表时间：
2021-03-11
影响因子：
4.6
通讯作者：
Nakano K
中科院分区：
文献类型：
--
作者：
Naka S;Wato K;Misaki T;Ito S;Matsuoka D;Nagasawa Y;Nomura R;Matsumoto-Nakano M;Nakano K
免疫球蛋白A肾病(IgAN)是最常见的慢性原发肾小球肾炎，其发病机制尚不清楚。变形链球菌是一种革兰氏阳性兼性厌氧口腔细菌，是导致龋齿的常见原因。在以往的研究中，在IgAN患者中经常检测到细胞表面表达CNM蛋白的变形链球菌。在本研究中，将CNM阳性变形链球菌接种于2周龄无特定病原体的SD大鼠的口腔中，喂养高糖饮食32周，在所有大鼠中都产生了严重的龋病。用IgA和补体C3特异性抗体对肾脏进行免疫组织化学分析，发现肾小球系膜区染色阳性。扫描电子显微镜显示肾小球系膜区有大量致密的电子沉积，高碘酸-席夫染色显示系膜细胞和系膜基质明显增殖。这些结果表明，CNM阳性变形链球菌可诱导严重龋病大鼠的IgAN样肾小球肾炎。
The mechanisms underlying immunoglobulin A nephropathy (IgAN), the most common chronic form of primary glomerulonephritis, remain poorly understood. Streptococcus mutans, a Gram-positive facultatively anaerobic oral bacterium, is a common cause of dental caries. In previous studies, S. mutans isolates that express Cnm protein on their cell surface were frequently detected in IgAN patients. In the present study, inoculation of Cnm-positive S. mutans in the oral cavities of 2-week-old specific-pathogen free Sprague–Dawley rats fed a high-sucrose diet for 32 weeks produced severe dental caries in all rats. Immunohistochemical analyses of the kidneys using IgA- and complement C3-specific antibodies revealed positive staining in the mesangial region. Scanning electron microscopy revealed a wide distribution of electron dense deposits in the mesangial region and periodic acid-Schiff staining demonstrated prominent proliferation of mesangial cells and mesangial matrix. These results suggest that IgAN-like glomerulonephritis was induced in rats with severe dental caries by Cnm-positive S. mutans.
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DOI：
10.1093/ndt/gfp273
发表时间：
2009-10
期刊：
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
影响因子：
--
作者：
Goto M;Wakai K;Kawamura T;Ando M;Endoh M;Tomino Y
通讯作者：
Tomino Y
影响因子：
4.6
作者：
Misaki T;Naka S;Hatakeyama R;Fukunaga A;Nomura R;Isozaki T;Nakano K
通讯作者：
Nakano K
影响因子：
8.6
作者：
Fujieda, S;Suzuki, S;Saito, H
通讯作者：
Saito, H
影响因子：
7.6
作者：
Keyes, P. H.
通讯作者：
Keyes, P. H.
影响因子：
2.5
作者：
HARA, M;YAMAMOTO, T;KIHARA, I
通讯作者：
KIHARA, I