Alizarin and Chrysazin Inhibit Biofilm and Hyphal Formation by Candida albicans.
Alizarin and Chrysazin Inhibit Biofilm and Hyphal Formation by Candida albicans.
复制标题
DOI：
10.3389/fcimb.2017.00447
复制
发表时间：
2017
影响因子：
5.7
通讯作者：
Lee J
中科院分区：
文献类型：
--
作者：
Manoharan RK;Lee JH;Kim YG;Lee J
白色念珠菌是引起真菌感染最常见的病原菌之一。该条件致病菌可形成由酵母菌、菌丝和假菌丝成分组成的生物膜，菌丝型C。白色念珠菌被认为是可能的毒力因子。本文研究了13种醌类及蒽醌类化合物对C.通过使用结晶紫和2，3-双（2-甲氧基-4-硝基-5-磺基-苯基）-2H-四唑鎓-5-甲酰苯胺（XTT）还原测定来评估生物膜生长的抑制。生物膜的形态变化和生物膜厚度分别通过扫描电子显微镜和共聚焦激光扫描显微镜测定。茜素（1，2-二羟基蒽醌）和金雀黄素（1，8-二羟基蒽醌）对C.白色念珠菌生物膜形成。2 μg/ml的茜素和金素能有效地抑制菌丝的形成，延长菌丝的存活时间。白色念珠菌感染秀丽隐杆线虫，从而显示出独特的抗毒性潜力。对茜素和其他6种蒽醌类化合物的构效关系研究表明，茜素的C-1位上存在一个羟基，这对茜素膜的形成和抗氧化活性具有重要意义。转录组学分析显示，茜素下调了几个菌丝特异性和生物膜相关基因（ALS 3，ECE 1，ECE 2和RBT 1）的表达。此外，与商业抗真菌药物氟康唑不同，当未感染的线虫暴露于浓度高达1 mg/ml的茜素时，未观察到急性毒性作用。本研究结果表明茜素对C.表明茜素可能被认为是进一步研究开发抗真菌病原体体内抗真菌剂的潜在候选物。
Candida albicans is one of the most common pathogen causes fungal infections. This opportunistic pathogen can form biofilms comprised of yeast, hyphae and pseudo hyphal elements, and the hyphal form C. albicans considered as probable virulence factor. We investigated the antibiofilm activities of 13 quinones and anthraquinones related compounds against C. albicans biofilms by using crystal violet and 2,3-bis (2-Methoxy-4-Nitro-5-Sulfo-phenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) reduction assays to assess inhibitions of biofilm growth. Morphological changes in biofilms and biofilm thicknesses were determined by scanning electron microscopy and confocal laser scanning microscopy, respectively. It was found alizarin (1,2-dihydroxyanthraquinone) and chrysazin (1,8-dihydroxyanthraquinone) suppressed C. albicans biofilm formation. Interestingly, alizarin and chrysazin at only 2 μg/ml effectively inhibited hyphal formation and prolonged the survival of C. albicans infected Caenorhabditis elegans, thus showing a distinct antivirulent potential. A structural activity relationship study of alizarin and 6 other anthraquinones showed the presence of a hydroxyl group at C-1 position which is important for antibiofilm and antifilamentation activities. Transcriptomic analyses revealed that alizarin downregulated the expression of several hypha-specific and biofilm related genes (ALS3, ECE1, ECE2, and RBT1). Furthermore, unlike the commercial antifungal drug fluconazole, no acute toxic effect was observed when uninfected nematodes were exposed to alizarin at concentrations up to 1 mg/ml. The results of this study indicate alizarin suppresses the virulence of C. albicans in vivo which suggests alizarin may be considered as a potential candidate for further investigations to develop antifungal agent against fungal pathogen in vivo.
登录
查看更多内容
影响因子：
2.6
作者：
Janeczko, Monika;Maslyk, Maciej;Golczyk, Hieronim
通讯作者：
Golczyk, Hieronim
影响因子：
3
作者：
Grald, Ariel;Yargosz, Philip;Johnson, Douglas I.
通讯作者：
Johnson, Douglas I.
DOI：
10.1080/14756366.2016.1180596
发表时间：
2016-12-01
影响因子：
5.6
作者：
Carradori, Simone;Chimenti, Paola;Angiolella, Letizia
通讯作者：
Angiolella, Letizia
影响因子：
4.6
作者：
Haque F;Alfatah M;Ganesan K;Bhattacharyya MS
通讯作者：
Bhattacharyya MS
DOI：
10.1155/2016/5647182
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Cardoso NN;Alviano CS;Blank AF;Romanos MT;Fonseca BB;Rozental S;Rodrigues IA;Alviano DS
通讯作者：
Alviano DS