Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells.
Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells.
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DOI：
10.1021/acsnano.2c02195
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发表时间：
2022-06-28
期刊：
影响因子：
17.1
通讯作者：
Elani, Yuval
中科院分区：
文献类型：
--
作者：
Zubaite, Greta;Hindley, James W.;Ces, Oscar;Elani, Yuval
人工细胞是由生物分子构建的最小结构，旨在模仿细胞的过程、行为和结构。细胞生命的一个几乎无处不在的特征是内部内容的空间组织。我们从生物学中知道，内容物的组织(包括在膜结合的细胞器中)与细胞功能有关，并且这一特征是动态的：其存在、位置和分隔程度随着时间的推移而变化。然而，基于囊泡的人工细胞目前还不能模仿这种基本的细胞特性。在这里，我们描述了一种解决这一技术瓶颈的人造细胞设计策略。我们创建了一系列人工细胞结构，这些人工细胞结构具有位于人工细胞膜内表面或外表面的多室组件。利用液-液相分离，我们还可以设计与细胞表面相连的浓缩亚室的空间隔离区域，使其与共存的膜域保持一致。这些结构可以感知环境条件的变化，并通过可逆地从膜表面的浓缩多室层过渡到细胞腔中的分散状态来响应，模仿生物细胞中发现的动态分区。同样，我们设计了可以释放到环境中的外周体样小室。我们可以通过使用两种类型的触发器来实现这一点：化学触发器(添加盐)和机械触发器(通过使用光学陷阱拉膜系绳)。这些方法使我们能够在群体和单细胞水平上控制人工细胞的区隔状态。
Artificial cells are minimal structures constructed from biomolecular building blocks designed to mimic cellular processes, behaviors, and architectures. One near-ubiquitous feature of cellular life is the spatial organization of internal content. We know from biology that organization of content (including in membrane-bound organelles) is linked to cellular functions and that this feature is dynamic: the presence, location, and degree of compartmentalization changes over time. Vesicle-based artificial cells, however, are not currently able to mimic this fundamental cellular property. Here, we describe an artificial cell design strategy that addresses this technological bottleneck. We create a series of artificial cell architectures which possess multicompartment assemblies localized either on the inner or on the outer surface of the artificial cell membrane. Exploiting liquid–liquid phase separation, we can also engineer spatially segregated regions of condensed subcompartments attached to the cell surface, aligning with coexisting membrane domains. These structures can sense changes in environmental conditions and respond by reversibly transitioning from condensed multicompartment layers on the membrane surface to a dispersed state in the cell lumen, mimicking the dynamic compartmentalization found in biological cells. Likewise, we engineer exosome-like subcompartments that can be released to the environment. We can achieve this by using two types of triggers: chemical (addition of salts) and mechanical (by pulling membrane tethers using optical traps). These approaches allow us to control the compartmentalization state of artificial cells on population and single-cell levels.
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影响因子：
2.9
作者：
Boettcher, John M.;Davis-Harrison, Rebecca L.;Clay, Mary C.;Nieuwkoop, Andrew J.;Ohkubo, Y. Zenmei;Tajkhorshid, Emad;Morrissey, James H.;Rienstra, Chad M.
通讯作者：
Rienstra, Chad M.
影响因子：
16.6
作者：
Bolognesi G;Friddin MS;Salehi-Reyhani A;Barlow NE;Brooks NJ;Ces O;Elani Y
通讯作者：
Elani Y
影响因子：
16.6
作者：
Elani, Yuval;Law, Robert V.;Ces, Oscar
通讯作者：
Ces, Oscar
影响因子：
17.1
作者：
Chandrawati, Rona;Hosta-Rigau, Leticia;Caruso, Frank
通讯作者：
Caruso, Frank
影响因子：
15
作者：
Deng, Nan-Nan;Yelleswarapu, Maaruthy;Huck, Wilhelm T. S.
通讯作者：
Huck, Wilhelm T. S.