Deuterium-depleted water (DDW) inhibits the proliferation and migration of nasopharyngeal carcinoma cells in vitro.
Deuterium-depleted water (DDW) inhibits the proliferation and migration of nasopharyngeal carcinoma cells in vitro.
复制标题
低氘水（DDW）在体外抑制鼻咽癌细胞的增殖和迁移。
DOI：
10.1016/j.biopha.2013.02.001
复制
发表时间：
2013-07
影响因子：
7.5
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Zhang X;Tian L;Fang W;Yang H
最近的研究表明，天然水具有65%的氘浓度耗尽，可以表现出抗肿瘤特性。然而，DDW对各种鼻咽癌（NPC）细胞的抗肿瘤作用以前未见报道。在本研究中，NPC细胞系和正常前成骨细胞MC 3 T3-E1细胞在含有不同氘浓度（50-150 ppm）的RPMI 1640培养基中生长。采用MTT法、平板集落形成法、Transwell法、Boyden小室阵列法、流式细胞术、免疫印迹法和免疫荧光等方法，观察DDW对鼻咽癌细胞和MC 3 T3-E1细胞增殖和迁移的影响。DDW对鼻咽癌细胞的增殖、集落形成、迁移和侵袭有明显的抑制作用。与此相反，正常前成骨细胞MC 3 T3-E1细胞的生长被促进时，他们在DDW的存在下培养。细胞周期分析显示，DDW使鼻咽癌细胞周期阻滞于G1/S期，S期细胞减少，G1期细胞明显增多。Western blot分析显示，DDW处理后，NADPH：醌氧化还原酶-1（NQO 1）的表达显著增加，而免疫荧光分析显示，DDW处理后，PCNA和基质金属蛋白酶9（MMP 9）的表达显著降低。这些结果表明，DDW是一种新型的，无毒的辅助治疗剂，通过诱导NQO 1的表达和引起细胞周期阻滞，以及降低PCNA和MMP 9的表达来抑制NPC细胞的增殖，迁移和侵袭。
Recent studies have demonstrated that natural water that has 65% of the deuterium concentration depleted, can exhibit anti-tumor properties. However, the anti-tumor effects of DDW on various nasopharyngeal carcinoma (NPC) cells have not previously been reported. In the present study, NPC cell lines and normal preosteoblast MC3T3-E1 cells were grown in RPMI1640 media containing different deuterium concentrations (50–150 ppm). The effects of DDW on the proliferation and migration of NPC and MC3T3-E1 cells were investigated using the MTT, plate colony formation, and Transwell assays, as well as Boyden chamber arrays, flow cytometry (FCM), western blot and immunofluorescence. We found that DDW was an effective inhibitor of NPC cell proliferation, plated colony formation, migration and invasion. In contrast, the growth of normal preosteoblast MC3T3-E1 cells was promoted when they were cultured in the presence of DDW. Cell cycle analysis revealed that DDW caused cell cycle arrest in the G1/S transition, reduced the number of cells in the S phase and significantly increased the population of cells in the G1 phase in NPC cells. Western blot analysis revealed that treatment with DDW significantly increased the expression of NADPH:quinone oxidoreductase-1 (NQO1), while immunofluorescence assay analysis revealed that treatment with DDW decreased the expression of PCNA and matrix metalloproteinase 9 (MMP9) in NPC cells. These results demonstrated that DDW is a novel, non-toxic adjuvant therapeutic agent that suppresses NPC cell proliferation, migration, and invasion by inducing the expression of NQO1 and causing cell cycle arrest, as well as decreasing PCNA and MMP9 expression.
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DOI：
10.1124/jpet.103.059477
发表时间：
2004-04-01
影响因子：
3.5
作者：
Han, YS;Shen, HM;Pan, SS
通讯作者：
Pan, SS
影响因子：
56.9
作者：
GROSS, PR;SPINDEL, W
通讯作者：
SPINDEL, W
DOI：
--
发表时间：
2004
期刊：
Romanian journal of physiology : physiological sciences
影响因子：
--
作者：
W. Bild;V. Năstasă;I. Haulica
通讯作者：
W. Bild;V. Năstasă;I. Haulica
影响因子：
2
作者：
J. Hartmann;Y. Bader;Z. Horváth;P. Saiko;M. Grusch;C. Illmer;S. Madlener;M. Fritzer-szekeres;Nicole Heller;R. Alken;T. Szekeres
通讯作者：
J. Hartmann;Y. Bader;Z. Horváth;P. Saiko;M. Grusch;C. Illmer;S. Madlener;M. Fritzer-szekeres;Nicole Heller;R. Alken;T. Szekeres
DOI：
--
发表时间：
1999
期刊：
--
影响因子：
--
作者：
L. Tan;Y. Sitoh;H. Tjia
通讯作者：
L. Tan;Y. Sitoh;H. Tjia