A novel, rapidly acquired and persistent spatial memory task that induces immediate early gene expression.
A novel, rapidly acquired and persistent spatial memory task that induces immediate early gene expression.
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DOI：
10.1186/1744-9081-6-35
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发表时间：
2010-07-02
期刊：
影响因子：
--
通讯作者：
Nalbantoglu J
中科院分区：
文献类型：
--
作者：
Feldman LA;Shapiro ML;Nalbantoglu J
Morris水迷宫任务是一种依赖于校园的学习和记忆测试，通常需要3天到2周的训练。这项任务被用来评估空间学习，并诱导海马体中已知对学习和记忆至关重要的基因的表达。方案中的一个主要警告是训练持续时间延长，并且难以评估动物在训练期间学习任务的时间。我们在这里介绍了一个浓缩版的任务，像传统的水迷宫任务，创建持久的校园依赖的空间认知地图和elastin基因表达学习。这种模式是为大鼠设计的，以快速获得一个依赖于校园的空间认知地图，并保持这种记忆至少24小时。为了实现这一目标，我们将可见和隐藏的训练试验穿插在一起，以集中的方式进行，因此训练最多需要15分钟。在每次训练试验期间，基于对平台的疲劳以及在训练后30分钟和24小时的探测测试期间在目标象限中花费的时间来评估学习。将正常大鼠与两个受损队列（穹窿海马伞损伤大鼠和给予NMDA受体拮抗剂（CPP）的大鼠）进行比较。为了定量已知学习基因的海马表达，对海马cDNA进行实时聚合酶链反应（RT-PCR）。我们发现，使用交替可见和隐藏训练试验的大规模训练在大鼠中产生了强大的短期工作记忆和长期参考记忆。与传统的Morris水迷宫范例一样，该任务需要适当的海马功能，因为穹窿海马伞损伤的大鼠和给予CPP的大鼠无法学习任务的空间成分。此外，在这种模式下的训练可以使海马基因的表达在学习各种空间任务后上调：homer 1a，cfos和zif 268。我们在这里介绍一个浓缩版的莫里斯水迷宫，这是像一个传统的水迷宫范例，因为它是依赖于海马体，和elastically海马表达的学习基因。然而，这项任务在15分钟内完成，并诱导至少24小时的空间记忆。
The Morris water maze task is a hippocampus-dependent learning and memory test that typically takes between 3 days to 2 weeks of training. This task is used to assess spatial learning and induces the expression of genes known to be crucial to learning and memory in the hippocampus. A major caveat in the protocol is the prolonged duration of training, and difficulty of assessing the time during training in which animals have learned the task. We introduce here a condensed version of the task that like traditional water maze tasks, creates lasting hippocampus-dependent spatial cognitive maps and elicits gene expression following learning. This paradigm was designed for rats to quickly acquire a hippocampus-dependent spatial cognitive map and retain this memory for at least 24 hours. To accomplish this, we interspersed visible and hidden training trials, delivering them in a massed fashion so training takes a maximum of 15 minutes. Learning was assessed based on latencies to the platform during each training trial, as well as time spent in the goal quadrant during probe testing 30 minutes and 24 hours after training. Normal rats were compared to two impaired cohorts (rats with fimbria-fornix lesions and rats administered NMDA receptor antagonist (CPP)). To quantitate hippocampal expression of known learning genes, real-time polymerase chain reaction (RT-PCR) was performed on hippocampal cDNA. We show that massed training using alternating visible and hidden training trials generates robust short-term working and long-term reference memories in rats. Like the traditional Morris water maze paradigm, this task requires proper hippocampal function, as rats with fimbria-fornix lesions and rats administered CPP fail to learn the spatial component of the task. Furthermore, training in this paradigm elicits hippocampal expression of genes upregulated following learning in a variety of spatial tasks: homer1a, cfos and zif268. We introduce here a condensed version of the Morris water maze, which is like a traditional water maze paradigm, in that it is hippocampus-dependent, and elicits hippocampal expression of learning genes. However, this task is administered in 15 minutes and induces spatial memory for at least 24 hours.
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