The secreted ribonuclease T2 protein FoRnt2 contributes to Fusarium oxysporum virulence.
The secreted ribonuclease T2 protein FoRnt2 contributes to Fusarium oxysporum virulence.
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DOI：
10.1111/mpp.13237
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发表时间：
2022-09
影响因子：
4.9
通讯作者：
Liang, Wenxing
中科院分区：
文献类型：
--
作者：
Qian, Hengwei;Wang, Lulu;Wang, Baoshan;Liang, Wenxing
仅从少数病原体中报道了分泌的RNase蛋白，对其生物学功能的了解相对较少。尖孢镰刀菌（Fusarium oxysporum）是引起番茄枯萎病（Fusarium wilt）的土传真菌病原体。在尖孢镰刀菌侵染过程中，会分泌一些调节寄主植物免疫，促进病原菌入侵的蛋白。在这项研究中，我们从尖孢镰刀菌分泌组中鉴定了一个核糖核酸酶T2家族的核糖核酸酶FoRnt2。fnt2具有N端信号肽，可由尖孢菌分泌。FoRnt2表现出核糖核酸酶活性，能够在体外降解寄主植物总RNA，依赖于活性位点残基H80和H142。FoRnt2基因的缺失降低了真菌的毒力，但对菌丝生长和分生孢子的产生没有明显影响。在番茄中表达FoRnt2可显著增强植株对病原菌的敏感性。这些数据表明，FoRnt2可能通过降解植物RNA对尖孢镰刀菌的毒力起重要作用。FoRnt2是尖孢镰刀菌（Fusarium oxysporum）分泌的核糖核酸酶T2家族的一员，能够降解宿主RNA，是土传真菌病原体毒力的重要贡献者。
Secreted RNase proteins have been reported from only a few pathogens, and relatively little is known about their biological functions. Fusarium oxysporum is a soilborne fungal pathogen that causes Fusarium wilt, one of the most important diseases on tomato. During the infection of F. oxysporum, some proteins are secreted that modulate host plant immunity and promote pathogen invasion. In this study, we identify an RNase, FoRnt2, from the F. oxysporum secretome that belongs to the ribonuclease T2 family. FoRnt2 possesses an N‐terminal signal peptide and can be secreted from F. oxysporum. FoRnt2 exhibited ribonuclease activity and was able to degrade the host plant total RNA in vitro dependent on the active site residues H80 and H142. Deletion of the FoRnt2 gene reduced fungal virulence but had no obvious effect on mycelial growth and conidial production. The expression of FoRnt2 in tomato significantly enhanced plant susceptibility to pathogens. These data indicate that FoRnt2 is an important contributor to the virulence of F. oxysporum, possibly through the degradation of plant RNA. FoRnt2, a member of the ribonuclease T2 family secreted from Fusarium oxysporum, is able to degrade host RNA and represents an important contributor to the virulence of the soilborne fungal pathogen.
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