Butanol Production from Wheat Straw by Combining Crude Enzymatic Hydrolysis and Anaerobic Fermentation Using Clostridium acetobutylicum ATCC824
Butanol Production from Wheat Straw by Combining Crude Enzymatic Hydrolysis and Anaerobic Fermentation Using Clostridium acetobutylicum ATCC824
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粗酶水解与丙酮丁醇梭菌 ATCC824 厌氧发酵相结合从小麦秸秆生产丁醇
DOI：
10.1021/ef4010658
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Qian Yang
中科院分区：
文献类型：
--
作者：
Zhenyu Wang;Guangli Cao;Cheng Jiang;Jinzhu Song;Ju Zheng;Qian Yang
本文研究了绿色木霉粗酶提取液对乙酰丁基梭菌ATCC824发酵生产丁醇的影响。发酵前，在50.7℃、pH 5.1、纤维素酶用量41.1IU/g、底物浓度38.1g/L的优化条件下，麦草粗酶解麦草，产糖量为17.32g/L，糖化率为82.2%。分离水解液发酵54h后，最终丁醇浓度、产率和最高产率分别为7.05g/L、0.155 g/g葡萄糖和0.141 g/L·h。同时进行了糖化和发酵试验，得到了丁醇浓度为5.05g/L、葡萄糖为0.127 g/g、L·h为0.080 g/g的较好结果。所得结果与用市售纤维素酶测得的结果相当，并具有明显的指标性。
In this study we investigated the use of a crude enzyme extract from Trichoderma viride to hydrolyze wheat straw for butanol production by Clostridium acetobutylicum ATCC824. Prior to fermentation, the wheat straw was hydrolyzed by the crude enzyme at the optimized conditions of 50.7 °C, pH 5.1, 41.1 IU/g cellulase dosage, and 38.1 g/L substrate concentration, in which the sugar yield was 17.32 g/L, resulting in a 82.2% saccharification efficiency of commercial cellulase. The final butanol concentration, yield, and maximum productivity, 7.05 g/L, 0.155 g/g of glucose, and 0.141 g/L·h, were observed in separated hydrolysis and fermentation after 54 h of fermentation using 2-fold concentrated hydrolysate. Simultaneous saccharification and fermentation was also investigated, and appreciable butanol concentration, yield, and productivity of 5.05 g/L, 0.127 g/g of glucose, and 0.080 g/L·h were attained. The results described here are comparable to those obtained by using commercial cellulase and clearly indica...
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