Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse.
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse.
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将人类脂肪来源的间充质干细胞接种到胶原羟基磷灰石支架中，在植入小鼠体内后可促进骨增强。
DOI：
10.1038/s41598-017-07672-0
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发表时间：
2017-08-02
影响因子：
4.6
通讯作者：
Gulino R
中科院分区：
文献类型：
--
作者：
Calabrese G;Giuffrida R;Forte S;Fabbi C;Figallo E;Salvatorelli L;Memeo L;Parenti R;Gulisano M;Gulino R
创伤性损伤或病变骨组织的手术切除通常需要重建无法自发愈合的大骨缺损，尤其是在老年人中。这是一个巨大的挑战，需要开发模拟骨结构的生物材料，并能够诱导接种在支架内的细胞的正确定向。特别是，鉴于其特性和大量可用性，人脂肪源性干细胞被认为是自体细胞移植的更好候选者。为了评价这些细胞沿着骨诱导生物材料的再生潜力，我们使用胶原/羟基磷灰石支架来测试小鼠皮下植入后的异位骨形成。通过荧光分子断层扫描（FMT）在体内和离体分析该过程，以评估骨和血管结构的形成。结果表明，生物材料本身可能能够促进宿主细胞的分化和骨形成，可能是通过其内在的化学和结构特性，即微环境。然而，当充入人间充质干细胞时，支架内的异位骨形成增加。我们相信这些结果代表了骨生理学领域以及再生医学领域的重要进展。
Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of inducing the right commitment of cells seeded within the scaffold. In particular, given their properties and large availability, the human adipose-derived stem cells are considered as the better candidate for autologous cell transplantation. In order to evaluate the regenerative potential of these cells along with an osteoinductive biomaterial, we have used collagen/hydroxyapatite scaffolds to test ectopic bone formation after subcutaneous implantation in mice. The process was analysed both in vivo, by Fluorescent Molecular Tomography (FMT), and ex vivo, to evaluate the formation of bone and vascular structures. The results have shown that the biomaterial could itself be able of promoting differentiation of host cells and bone formation, probably by means of its intrinsic chemical and structural properties, namely the microenvironment. However, when charged with human mesenchymal stem cells, the ectopic bone formation within the scaffold was increased. We believe that these results represent an important advancement in the field of bone physiology, as well as in regenerative medicine.
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胶原 - 羟基磷灰石支架在体外诱导人脂肪衍生的干细胞成骨分化。
影响因子：
3.7
作者：
Calabrese G;Giuffrida R;Fabbi C;Figallo E;Lo Furno D;Gulino R;Colarossi C;Fullone F;Giuffrida R;Parenti R;Memeo L;Forte S
通讯作者：
Forte S
影响因子：
4.9
作者：
Pasqui, Daniela;Torricelli, Paola;Barbucci, Rolando
通讯作者：
Barbucci, Rolando
DOI：
10.1073/pnas.1119474109
发表时间：
2012-05-15
影响因子：
11.1
作者：
Mandal, Biman B.;Grinberg, Ariela;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
14
作者：
Fricain, Jean Christophe;Schlaubitz, Silke;Amedee, Joelle
通讯作者：
Amedee, Joelle
DOI：
10.1097/01.blo.0000165849.32661.5e
发表时间：
2005-06-01
影响因子：
4.2
作者：
Joyce, MJ
通讯作者：
Joyce, MJ