In vitro and in vivo study of the pathogenic role of PPARα in experimental periodontitis.
In vitro and in vivo study of the pathogenic role of PPARα in experimental periodontitis.
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DOI：
10.1590/1678-7757-2022-0076
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发表时间：
2022
影响因子：
2.7
通讯作者：
Hu, Yang
中科院分区：
文献类型：
--
作者：
Chen, Ying;Jiang, Zheqing;Keohane, Ana;Hu, Yang
本研究的目的是探讨PPARα对牙周抗原处理牙龈细胞和体内实验性牙周炎的致病作用。从C57BL/6J野生型（WT）小鼠中分离牙龈成纤维细胞、牙龈上皮细胞和脾细胞，用固定的牙龈假单胞菌处理48小时。Real-time定量PCR检测PPARs、TNFα、IL-1β和IL-10 mRNA水平。将丝结扎物浸泡在pginivalis悬浮液中，在WT小鼠或PPARα敲除（KO）小鼠的上颌第二磨牙周围结扎2周。PPARα激动剂非诺贝特和对照物分别于第3、6和9天注射于腭龈的不同侧。第14天，分别采用显微计算机断层扫描和RT-qPCR检测骨吸收和牙龈PPARs、TNFα、IL-1β和IL-10 mRNA表达水平。P. gingivalis可下调WT小鼠牙龈成纤维细胞、牙龈上皮细胞和脾细胞中PPARα的表达，而不下调PPARβ和PPARγ的表达，并可上调TNF-α和IL-1β的表达。实验性牙周炎小鼠牙龈PPARα mRNA水平明显降低。实验性牙周炎PPARα KO小鼠的骨质流失明显高于WT小鼠，非诺贝特治疗并未减少骨质流失。在WT小鼠中，牙龈卟啉卟啉结扎显著增加了牙龈TNFα蛋白的表达，非诺贝特治疗显著降低了牙龈TNFα蛋白的表达，而在PPARα KO小鼠中则没有。本研究提示PPARα在牙周炎中起重要作用。
The purpose of this study is to investigate the pathogenic role of PPARα in periodontal antigen treated gingival cells in vitro and in experimental periodontitis in vivo . Gingival fibroblasts, gingival epithelial cells and splenocytes were isolated from C57BL/6J wild type (WT) mice and treated with fixed P. gingivalis at for 48 hours. The mRNA levels of PPARs, TNFα, IL-1β and IL-10 were detected by Real-time quantitative PCR. Silk ligatures after being soaked in the P.gingivalis suspension were tied around both maxillary second molars of WT mice or PPARα knock-out (KO) mice for two weeks. PPARα agonist fenofibrate and vehicle control were injected into the different side of the palatal gingiva on days 3, 6, and 9. At day 14, bone resorption and gingival mRNA expression levels of PPARs, TNFα, IL-1β and IL-10 were measured by micro-computed tomography and RT-qPCR respectively. P. gingivalis treatment downregulated the expression of PPARα, but not PPARβ or PPARγ, and increased the expression of TNF-α and IL-1β in Gingival fibroblasts, gingival epithelial cells and splenocytes from WT mice. Gingival mRNA levels of PPARα were significantly decreased in experimental periodontitis in WT mice. The bone loss of PPARα KO mice in experimental periodontitis was significantly higher than WT mice and was not reduced by fenofibrate treatment. Gingival TNFα protein expressions were significantly increased by P. gingivalis associated ligation and decreased by fenofibrate treatment in WT mice but not in PPARα KO mice. This study suggests that PPARα plays an essential role in periodontitis.
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影响因子：
--
作者：
Díaz-Gandarilla JA;Osorio-Trujillo C;Hernández-Ramírez VI;Talamás-Rohana P
通讯作者：
Talamás-Rohana P
影响因子：
3.3
作者：
Abbott, Barbara D.
通讯作者：
Abbott, Barbara D.
影响因子：
15.9
作者：
Akune, T;Ohba, S;Kawaguchi, H
通讯作者：
Kawaguchi, H
影响因子：
6.7
作者：
Gruber, Reinhard
通讯作者：
Gruber, Reinhard
DOI：
10.1590/1678-7757-2021-0648
发表时间：
2022
期刊：
Journal of applied oral science : revista FOB
影响因子：
--
作者：
Chen Y;Hu Y
通讯作者：
Hu Y