Identification and characterization of microsatellites in Aconitum reclinatum (Ranunculaceae), a rare species endemic to North America.
Identification and characterization of microsatellites in Aconitum reclinatum (Ranunculaceae), a rare species endemic to North America.
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北美洲特有稀有物种乌头（毛茛科）微卫星的识别和表征
DOI：
10.1002/aps3.1161
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发表时间：
2018-06
影响因子：
3.6
通讯作者：
Gong W
中科院分区：
文献类型：
--
作者：
Zhou J;Liu W;Kong H;Gong W
aconitum肿块是北美的唯一代表性物种。
使用Illumina HISEQ技术，我们对简单的序列重复标记进行了测序。在人口下，平均观察到的杂合水平分别为0至1.000和0在四个密切相关的物种中，三个底漆对成功进行了扩增。
在这项研究中分离的微卫星将对北美颗粒状人群的遗传多样性和保护遗传学的进一步研究有用。
Premise of the Study Aconitum reclinatum is the only representative species of Aconitum subg. Lycoctonum in North America, with restricted ranges and endangered populations. Polymorphic microsatellite markers were developed for A. reclinatum for further investigation of genetic diversity and population structure. Methods and Results Using Illumina HiSeq technology, we sequenced a genomic library for identification of simple sequence repeat markers. A total of 12 polymorphic primer pairs were developed and tested on 66 individuals from four populations in North America. The number of alleles ranged from one to seven per locus with an average of 3.48. Levels of observed and expected heterozygosity varied from 0 to 1.000 and 0 to 0.736, respectively, at population level. Three primer pairs were successfully amplified in three of four closely related species. Conclusions The microsatellites isolated in this study will be useful in further research on the genetic diversity and conservation genetics of A. reclinatum populations in North America.
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影响因子：
4.1
作者：
Jabbour, Florian;Renner, Susanne S.
通讯作者：
Renner, Susanne S.
影响因子：
--
作者：
BRINK, D
通讯作者：
BRINK, D
影响因子：
3.6
作者：
Ge XY;Tian H;Liao WJ
通讯作者：
Liao WJ
DOI：
10.1111/j.1471-8286.2004.00684.x
发表时间：
2004-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Van Oosterhout, C;Hutchinson, WF;Shipley, P
通讯作者：
Shipley, P
影响因子：
1.1
作者：
He, Jun;Zhang, Zhi-Rong;Meng, Jing
通讯作者：
Meng, Jing