Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis
Cloning and expression analysis on a homolog of spermatogonial stem-cell renewal factor in Fenneropenaeus chinensis
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中国明对虾精原干细胞更新因子同源物的克隆及表达分析
DOI：
10.1080/07924259.2014.905501
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发表时间：
2014-06
影响因子：
0.8
通讯作者：
Jianhai Xiang
中科院分区：
文献类型：
--
作者：
Shihao Li;Chengsong Zhang;Kuijie Yu;Jianhai Xiang
精原干细胞更新因子（SSRF）因其在日本鳗鲡精原细胞有丝分裂中的作用而被命名。我们前期的研究表明，SSRF同源基因在三倍体对虾的卵巢中高表达，而在二倍体对虾的卵巢中不表达。为了解SSRF在对虾中的功能，从中国对虾Fenneropenaeus chinensis （fssrf）中克隆了SSRF基因的全长cDNA，并对其表达进行了分析。fssrf cDNA全长2588 bp，包含一个开放阅读框，编码450个氨基酸。预测的fssrf三级结构与日本鳗鲡的SSRF/eSRS34和智人的TP/PD-ECGF非常相似。RT-PCR分析显示，fssrf在神经、睾丸、肝胰腺、鳃和胃中高表达，而在卵巢中不高表达。在二倍体和三倍体虾的胚胎期和幼虫期，从幼体到幼虫后期，均未检测到fssrf mRNA的表达。然而，在二倍体和三倍体虾的幼期（6 - 9月）开始表达。免疫组化分析显示，fssrf在二倍体睾丸和三倍体卵巢中均有表达。我们推测fssrf可能与睾丸发育有关。
The spermatogonial stem-cell renewal factor (SSRF) was named since its function in spermatogonial mitosis was reported in Japanese eel. Our previous study showed that a homolog of SSRF was highly expressed in the ovary of triploid shrimp, but not expressed in the ovary of diploid shrimp. To understand the function of SSRF in shrimp, the full-length cDNA of ssrf gene was cloned from Chinese shrimp Fenneropenaeus chinensis (Fcssrf) and its expression was analyzed. The full length of Fcssrf cDNA was 2588 bp and it contained an open reading frame encoding 450 amino acids. The predicted tertiary structure of FcSSRF was very similar to that of SSRF/eSRS34 from Anguilla japonica and TP/PD-ECGF from Homo sapiens. RT-PCR analysis showed that the Fcssrf was highly expressed in nerve, testis, hepatopancreas, gill, and stomach rather than in ovary. Expression of Fcssrf mRNA was not detected during embryonic stages and larval stages, from the nauplii to the post-larvae stage, in diploid, and triploid shrimp. However, it began to be expressed in juvenile stages (June–September) in diploid and triploid shrimp. Immunohistochemical analyses showed that FcSSRF was identified in both the diploid testis and triploid ovary. We inferred that the Fcssrf might be related to testis development.
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影响因子：
2.5
作者：
P. Eccleston;K. Funa;C. Heldin
通讯作者：
P. Eccleston;K. Funa;C. Heldin
影响因子：
4.8
作者：
Miura, T;Ohta, T;Yamauchi, K
通讯作者：
Yamauchi, K
影响因子：
4.5
作者：
Fuhua Li;J. Xiang;Linghua Zhou;Changgong Wu;Xiaojun Zhang
通讯作者：
Fuhua Li;J. Xiang;Linghua Zhou;Changgong Wu;Xiaojun Zhang
DOI：
--
发表时间：
1992-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Asai;K. Nakanishi;I. Isobe;Y. Eksioglu;A. Hirano;K. Hama;T. Miyamoto;T. Kato
通讯作者：
K. Asai;K. Nakanishi;I. Isobe;Y. Eksioglu;A. Hirano;K. Hama;T. Miyamoto;T. Kato
影响因子：
9.7
作者：
Fujimoto, J;Ichigo, S;Tamaya, T
通讯作者：
Tamaya, T