Combining Visible Light and Non-Focused Ultrasound Significantly Reduces Propionibacterium acnes Biofilm While Having Limited Effect on Host Cells.
Combining Visible Light and Non-Focused Ultrasound Significantly Reduces Propionibacterium acnes Biofilm While Having Limited Effect on Host Cells.
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DOI：
10.3390/microorganisms9050929
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发表时间：
2021-04-26
期刊：
影响因子：
4.5
通讯作者：
McNeely T
中科院分区：
文献类型：
--
作者：
Schafer ME;McNeely T
细菌生物膜对抗生素具有高度耐药性，并且与60%-80%的慢性微生物感染的病因学有关。我们测试了一种新的低强度超声波和蓝光的组合对生物膜和浮游细菌。建立了一个实验室原型，它从一个单一的治疗头均匀和一致地产生两种能量，撞击在4.45平方厘米的目标。为了证明概念的证明，将痤疮丙酸杆菌生物膜在6孔板中的Millicell悬挂插入物上培养。在定制的暴露室中处理具有生物膜的悬挂插入物，所述暴露室被设计成最小化不需要的超声反射。两种能量的同时递送证明了与单独的任一种相比的协同作用，杀死痤疮丙酸杆菌的静止增殖和生物膜培养物。生物膜细菌的减少是剂量依赖于暴露时间（即，能量传递）。痤疮丙酸杆菌生物膜通过双能量处理显著减少（p < 0.0001），在5分钟（9 J/cm 2）后减少>1 log 10，并且在30分钟（54 J/cm 2）处理后减少>3 log 10（p < 0.05）。发现哺乳动物细胞不受处理影响。光和超声波能量都在FDA先前批准的水平。因此，这种联合治疗可以用作治疗生物膜相关综合征的安全、有效的方法。
Bacterial biofilms are highly resistant to antibiotics and have been implicated in the etiology of 60%–80% of chronic microbial infections. We tested a novel combination of low intensity ultrasound and blue light against biofilm and planktonic bacteria. A laboratory prototype was built which produced both energies uniformly and coincidently from a single treatment head, impinging upon a 4.45 cm2 target. To demonstrate proof of concept, Propionibacterium acnes biofilms were cultured on Millicell hanging inserts in 6-well plates. Hanging inserts with biofilms were treated in a custom exposure chamber designed to minimize unwanted ultrasound reflections. Coincident delivery of both energies demonstrated synergy over either alone, killing both stationary planktonic and biofilm cultures of P. acnes. Reduction in biofilm bacteria was dose dependent on exposure time (i.e., energy delivered). P. acnes biofilms were significantly reduced by dual energy treatment (p < 0.0001), with a >1 log10 reduction after a 5 min (9 J/cm2) and >3 log10 reduction after a 30 min (54 J/cm2) treatment (p < 0.05). Mammalian cells were found to be unaffected by the treatment. Both the light and the ultrasound energies are at levels previously cleared by the FDA. Therefore, this combination treatment could be used as a safe, efficacious method to treat biofilm related syndromes.
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