Bacterial multispecies interaction mechanisms dictate biogeographic arrangement between the oral commensals Corynebacterium matruchotii and Streptococcus mitis.
Bacterial multispecies interaction mechanisms dictate biogeographic arrangement between the oral commensals Corynebacterium matruchotii and Streptococcus mitis.
复制标题
DOI：
10.1128/msystems.00115-23
复制
发表时间：
2023-10-26
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
多微生物生物膜存在于许多环境中，特别是人类口腔中，它们可以预防或促进疾病的发生。虽然最近的进展提供了一个清晰的画面的成分和它们的地理安排，它仍然不清楚是什么机制的相互作用发生在这些社区内的个别物种之间的密切关系。在这项研究中，我们研究了两种机制之间的相互作用的高度丰富的龈上菌斑（SUPP）的马鲁霍氏棒状杆菌和缓症链球菌直接相邻/附着在体内。我们发现C. matruchotii增强了链球菌的适应性，这取决于它对链球菌产生的过氧化氢的解毒能力和它对也由链球菌产生的乳酸的氧化能力。我们证明了相邻链球菌的适应性与C. matruchotii，并且这些机制支持先前描述的这些物种之间的“玉米芯”排列，但是这仅在有氧条件下是有利的。此外，我们首次利用扫描电化学显微镜定量单个细菌细胞之间的乳酸产生和消耗，揭示了乳酸氧化为S.缓解不是由于pH值缓解。这项研究描述了两种高度丰富的人类细菌之间的相互作用机制，可以解释它们在体内观察到的空间排列，并提出了一种方法，通过这种方法，它们可能有助于保护健康的口腔细菌群落。随着微生物组时代的成熟，物种之间的机械相互作用数据对于了解如何保持稳定的微生物组至关重要，特别是在健康条件下，微生物群可以帮助抵抗机会性或外源性病原体。在这里，我们揭示了多种机制之间的相互作用，两个牙菌斑，决定他们的牙印相互关系，在以前描述的结构，在人类龈上菌斑。使用一种新的化学检测的变化，我们观察到代谢物交换个别细菌细胞在真实的时间验证这些生物体进行代谢交叉喂养在体内观察到的远端和时间尺度的能力。这些发现揭示了一种方式，通过这种方式，这些相互作用既有利于相互作用的宿主，也可能有利于宿主。
Polymicrobial biofilms are present in many environments particularly in the human oral cavity where they can prevent or facilitate the onset of disease. While recent advances have provided a clear picture of both the constituents and their biogeographic arrangement, it is still unclear what mechanisms of interaction occur between individual species in close proximity within these communities. In this study, we investigated two mechanisms of interaction between the highly abundant supragingival plaque (SUPP) commensal Corynebacterium matruchotii and Streptococcus mitis which are directly adjacent/attached in vivo. We discovered that C. matruchotii enhanced the fitness of streptococci dependent on its ability to detoxify streptococcal-produced hydrogen peroxide and its ability to oxidize lactate also produced by streptococci. We demonstrate that the fitness of adjacent streptococci was linked to that of C. matruchotii and that these mechanisms support the previously described “corncob” arrangement between these species but that this is favorable only in aerobic conditions. Furthermore, we utilized scanning electrochemical microscopy to quantify lactate production and consumption between individual bacterial cells for the first time, revealing that lactate oxidation provides a fitness benefit to S. mitis not due to pH mitigation. This study describes mechanistic interactions between two highly abundant human commensals that can explain their observed in vivo spatial arrangements and suggest a way by which they may help preserve a healthy oral bacterial community. As the microbiome era matures, the need for mechanistic interaction data between species is crucial to understand how stable microbiomes are preserved, especially in healthy conditions where the microbiota could help resist opportunistic or exogenous pathogens. Here we reveal multiple mechanisms of interaction between two commensals that dictate their biogeographic relationship to each other in previously described structures in human supragingival plaque. Using a novel variation for chemical detection, we observed metabolite exchange between individual bacterial cells in real time validating the ability of these organisms to carry out metabolic crossfeeding at distal and temporal scales observed in vivo. These findings reveal one way by which these interactions are both favorable to the interacting commensals and potentially the host.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.2
作者：
Brown, Stacie A.;Whiteley, Marvin
通讯作者：
Whiteley, Marvin
影响因子：
3.7
作者：
Abranches J;Zeng L;Kajfasz JK;Palmer SR;Chakraborty B;Wen ZT;Richards VP;Brady LJ;Lemos JA
通讯作者：
Lemos JA
影响因子：
2.6
作者：
Ankri, S;Reyes, O;Leblon, G
通讯作者：
Leblon, G