Expression of CK-19, galectin-3 and HBME-1 in the differentiation of thyroid lesions: systematic review and diagnostic meta-analysis.
Expression of CK-19, galectin-3 and HBME-1 in the differentiation of thyroid lesions: systematic review and diagnostic meta-analysis.
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DOI：
10.1186/1746-1596-7-97
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发表时间：
2012-08-13
影响因子：
2.6
通讯作者：
da Silva Pinhal MA
中科院分区：
文献类型：
--
作者：
de Matos LL;Del Giglio AB;Matsubayashi CO;de Lima Farah M;Del Giglio A;da Silva Pinhal MA
由于术前应用的细针穿刺活检方法有一定的局限性，因此，鉴别甲状腺良恶性病变往往需要组织学证实。为了提高诊断准确性，已经研究了使用免疫细胞化学和免疫组织化学技术的标志物，主要是细胞角蛋白-19（CK-19）、半乳糖凝集素-3（Gal-3）和Hector Battifora mesothelium-1（HBME-1）。然而，目前的结果仍然存在争议。本研究的目的是建立CK-19，Gal-3和HBME-1标记物的诊断准确性，以及它们之间的关联，在甲状腺良恶性病变的鉴别。对MEDLINE和科克伦图书馆发表的文章进行了系统综述。在确定纳入和排除标准后，选择了66篇文章。采用诊断准确性的荟萃分析技术，计算敏感性、特异性、ROC曲线下面积和诊断优势比（dOR）的总体值。对于免疫组化技术，CK-19诊断甲状腺恶性病变的阳性率显示出81%的敏感性和73%的特异性;对于Gal-3，敏感性为82%，特异性为81%;对于HBME-1，敏感性为77%，特异性为83%。三种标记物的关联确定了85%的灵敏度，97%的特异性和95.1的诊断优势比。免疫细胞化学检测也发现了类似的结果。这项荟萃分析表明，研究的三种免疫标记物在术前和术后诊断甲状腺良恶性病变方面是准确的。然而，必须继续寻找其他分子标记物，以提高诊断的准确性，因为发现的结果仍然显示假阴性和假阳性试验的持续性。Http://www.diagnosticpathology.diagnomx.eu/vs/3436263067345159
To distinguish between malignant and benign lesions of the thyroid gland histological demonstration is often required since the fine-needle aspiration biopsy method applied pre-operatively has some limitations. In an attempt to improve diagnostic accuracy, markers using immunocytochemistry and immunohistochemistry techniques have been studied, mainly cytokeratin-19 (CK-19), galectin-3 (Gal-3) and Hector Battifora mesothelial-1 (HBME-1). However, current results remain controversial. The aim of the present article was to establish the diagnostic accuracy of CK-19, Gal-3 and HBME-1 markers, as well as their associations, in the differentiation of malignant and benign thyroid lesions. A systematic review of published articles on MEDLINE and The Cochrane Library was performed. After establishing inclusion and exclusion criteria, 66 articles were selected. The technique of meta-analysis of diagnostic accuracy was employed and global values of sensitivity, specificity, area under the summary ROC curve, and diagnostic odds ratio (dOR) were calculated. For the immunohistochemistry technique, the positivity of CK-19 for the diagnosis of malignant thyroid lesions demonstrated global sensitivity of 81% and specificity of 73%; for Gal-3, sensitivity of 82% and specificity of 81%; and for HBME-1, sensitivity of 77% and specificity of 83%. The association of the three markers determined sensitivity of 85%, specificity of 97%, and diagnostic odds ratio of 95.1. Similar results were also found for the immunocytochemistry assay. This meta-analysis demonstrated that the three immunomarkers studied are accurate in pre- and postoperative diagnosis of benign and malignant thyroid lesions. Nevertheless, the search for other molecular markers must continue in order to enhance this diagnostic accuracy since the results found still show a persistency of false-negative and false-positive tests. Http://www.diagnosticpathology.diagnomx.eu/vs/3436263067345159
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影响因子：
6.4
作者：
Coli, A;Bigotti, G;Massi, G
通讯作者：
Massi, G
影响因子：
168.9
作者：
Bartolazzi, A;Gasbarri, A;Larsson, O
通讯作者：
Larsson, O
影响因子：
3.5
作者：
Galusca, B;Dumollard, JM;Peoc'h, M
通讯作者：
Peoc'h, M
DOI：
10.2310/7070.2003.11429
发表时间：
2003-10-01
期刊：
JOURNAL OF OTOLARYNGOLOGY
影响因子：
--
作者：
Cameron, BR;Berean, KW
通讯作者：
Berean, KW
影响因子：
5.6
作者：
Gaffney, RL;Carney, JA;Lloyd, RV
通讯作者：
Lloyd, RV