Expression of calcyclin in human melanoma cell lines correlates with metastatic behavior in nude mice.
Expression of calcyclin in human melanoma cell lines correlates with metastatic behavior in nude mice.
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人黑色素瘤细胞系中钙周期蛋白的表达与裸鼠的转移行为相关。
DOI：
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发表时间：
1992
期刊：
影响因子：
11.2
通讯作者：
Henri P. J. Bloomers
中科院分区：
文献类型：
--
作者：
Marian A. J. Weterman;Geert M. Stoopen;G. V. Muijen;Jacek Kuznicki;D J Ruiter;Henri P. J. Bloomers
由于我们的目标是分离和鉴定人类皮肤黑色素瘤的新的进展标记，我们应用了差异杂交技术，在该技术中，我们比较了这种进展的两个后续阶段的基因表达。本实验采用裸鼠移植肿瘤模型，对同一患者的原发黑色素瘤的水平生长部分、早期垂直生长部分或晚期垂直生长部分进行体内连续传代。这导致分离了许多差异表达的互补DNA克隆。根据显著的表达差异，其中一个被命名为pMW1的基因被选中进行进一步的鉴定，似乎编码的是钙周期蛋白，一种细胞周期调节蛋白，属于一个小的钙结合蛋白家族。在裸鼠体内的高转移人黑色素瘤细胞系中，与低转移黑色素瘤细胞系相比，钙周期蛋白的表达增加。钙周期蛋白的免疫沉淀结果表明，在RNA水平的差异表达也反映在蛋白质水平。这些发现表明，钙周期蛋白的表达与人黑色素瘤细胞系在裸鼠体内的转移有关，并强调了这一钙结合蛋白家族在肿瘤进展中的作用，就像在小鼠中报道的钙周期蛋白同源物和该家族的其他成员一样。
Since our aim was to isolate and identify new progression markers of human cutaneous melanoma, we applied the differential hybridization technique, in which we compared the gene expression in two subsequent stages of this progression. Tumors in nude mice arising after transplantation and serial passage in vivo of either the horizontally and early vertically growing part or the advanced vertically growing part of a primary melanoma of the same patient were used for this assay. This resulted in the isolation of a number of complementary DNA clones that were differentially expressed. Based on the marked difference in expression, one of them, designated pMW1, was chosen for further characterization and appeared to be coding for calcyclin, a cell cycle-regulated protein, belonging to a family of small calcium-binding proteins. Calcyclin expression was elevated in high-metastatic human melanoma cell lines in nude mice compared to low-metastatic ones. Immunoprecipitation of calcyclin showed that the differential expression at the RNA level is also reflected at the protein level. These findings show that expression of calcyclin is related to metastasis of human melanoma cell lines in nude mice and emphasize the role of this family of calcium-binding proteins in neoplastic progression as was reported for the mouse homologue of calcyclin and other members of the same family.
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影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
DOI：
10.1016/s0021-9258(18)47567-9
发表时间：
1987-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Ferrari;B. Calabretta;J. K. Deriel;R. Battini;F. Ghezzo;E. Lauret;C. Griffin;B. Emanuel;F. Gurrieri;R. Baserga
通讯作者：
S. Ferrari;B. Calabretta;J. K. Deriel;R. Battini;F. Ghezzo;E. Lauret;C. Griffin;B. Emanuel;F. Gurrieri;R. Baserga
影响因子：
3.3
作者：
CLARK, WH;ELDER, DE;VANHORN, M
通讯作者：
VANHORN, M
影响因子：
11.2
作者：
R. Becher;Z. Gibas;C. Karakousis;A. Sandberg
通讯作者：
R. Becher;Z. Gibas;C. Karakousis;A. Sandberg
影响因子：
14.9
作者：
JACKSONGRUSBY, LL;SWIERGIEL, J;LINZER, DIH
通讯作者：
LINZER, DIH