Preparation, structure and BMP-2 controlled release of heparin-conjugated hyaluronan microgels
Preparation, structure and BMP-2 controlled release of heparin-conjugated hyaluronan microgels
复制标题
肝素偶联透明质酸微凝胶的制备、结构及BMP-2控释
DOI：
10.1016/j.carbpol.2011.05.026
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发表时间：
2011-08
影响因子：
11.2
通讯作者：
赵剑豪
中科院分区：
文献类型：
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作者：
赵剑豪
通过反相乳液聚合技术合成了不同肝素含量（1％、5％和10％（w/w））的肝素偶联透明质酸（HA-Hp）微凝胶，用于控制释放骨形态发生蛋白-2（BMP-2）。通过元素分析获得约90％的肝素结合百分比。透明质酸微凝胶表现出光滑的表面和致密的网络，而HA-Hp微凝胶则随着肝素含量的增加，表现出更粗糙的表面，有孔和凹面，内部结构更疏松。然而，约3μm的主要微凝胶尺寸与肝素的量无关。其中，HA-Hp-10%微凝胶由于其交联网络最少，具有最高的平衡溶胀比，为11.8。随着肝素含量的增加，BMP-2 负载效率更高，释放速度更慢，表明 HA-Hp 微凝胶中的缀合肝素有利于 BMP-2 结合，持续递送可能归因于肝素和 BMP-2 之间的静电相互作用。
Heparin-conjugated hyaluronan (HA-Hp) microgels with different heparin content, i.e., 1%, 5% and 10% (w/w), were synthesized by an inverse emulsion polymerization technique for the controlled release of bone morphogenetic protein-2 (BMP-2). A heparin conjugation percentage of about 90% was obtained by elemental analysis. Hyaluronan microgels showed a smooth surface and dense network, whereas HA-Hp microgels exhibited a rougher surface with holes and concaves, and a looser internal structure with increasing the heparin content instead. However, the major microgel size of about 3μm was independent of the heparin amount. Among the samples, HA-Hp-10% microgels existed the highest equilibrium swelling ratio of 11.8 due to its least cross-linking network. A higher BMP-2 loading efficiency and a slower release profile with increasing the heparin content indicated the conjugated heparin in HA-Hp microgels was in favor of BMP-2 binding and the sustained delivery maybe attributed to the electrostatic interaction between heparin and BMP-2.
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影响因子：
4.1
作者：
Krawczak, David A.;Westendorf, Jennifer J.;Lewis, Jack L.
通讯作者：
Lewis, Jack L.
用于骨组织工程的壳聚糖纳米复合物的制备、结构、药物释放和仿生矿化
影响因子：
11.2
作者：
Liu, Hongwei;Zhao, Jianhao;Zha, Zhengang;Zhou, Changren
通讯作者：
Zhou, Changren
影响因子：
9.7
作者：
J. Zhang;Q. Wang;A. Wang
通讯作者：
J. Zhang;Q. Wang;A. Wang
影响因子：
5.6
作者：
GOSPODAROWICZ, D;CHENG, J
通讯作者：
CHENG, J
DOI：
10.1007/s10856-008-3566-3
发表时间：
2009
期刊：
Journal of Materials Science: Materials in Medicine
影响因子：
--
作者：
Barbara Zavan;Vincenzo Vindigni;K. Vezzù;G. Zorzato;C. Luni;Giovanni Abatangelo;N. Elvassore;R. Cortivo
通讯作者：
Barbara Zavan;Vincenzo Vindigni;K. Vezzù;G. Zorzato;C. Luni;Giovanni Abatangelo;N. Elvassore;R. Cortivo